分析化学2017，Vol.45Issue(7)：1038-1044,7.DOI:10.11895/j.issn.0253.3820.170085
表面电荷对聚乙二醇修饰金纳米粒子生物行为的影响
Effect of Surface Charge on Biobehavior ofPEGylated Gold Nanoparticles
摘要
Abstract
Three kinds of PEGylated gold nanoparticles (PEG-Au NPs) with different surface charges are prepared by assembly of thiolated polyethylene glycol (HS-PEG) with different terminal groups including methoxy, amino or carboxyl on gold nanoparticle surface through sulfur-gold covalent bond, respectively.The experimental results of cell co-culturing and tail intravenous injection in mouse indicate that the biological behaviors of PEG-Au NPs are affected significantly by their surface charges.The cellular internalization amounts of PEG-Au NPs are following the order, positive charge ＞ neutral charge ＞ negative charge.The PEG-Au NPs are gradually transferred to liver and spleen from main organs through the circulation of blood after tail intravenous injection in mouse.The negatively charged PEG-Au NPs have the slowest hepatic clearance rate while the positively charged PEG-Au NPs can cause the strongest response of immune system in mice.关键词
聚乙二醇/金纳米粒子/活细胞/活体分布/生物相容性Key words
PEGylated/Gold nanoparticles/Living cells/Biodistribution/Biocompatibility.引用本文复制引用
付宇,李晓东,李铸衡,陈宏达,王振新,张惠茅,王海峰..表面电荷对聚乙二醇修饰金纳米粒子生物行为的影响[J].分析化学,2017,45(7):1038-1044,7.基金项目
本文系国家自然科学基金项目(No.81571737)资助 This work was supported by the National Natural Science Foundation of China (No.81571737). （No.81571737）
