广东医学2017，Vol.38Issue(11)：1668-1670,3.
右美托咪定对大鼠体外循环期间炎性因子、S100β和NSE的影响
Effects of dexmedetomidine on inflammatory cytokines, S100β and NSE in rats undergoing cardiopulmonary bypass
摘要
Abstract
Objective To evaluate the effects of dexmedetomidine (Dex) on inflammatory cytokines,S100β and NSE in rats undergoing cardiopulmonary bypass.Methods Forty Sprague-Dawley rats,weighing 250 ～350 g,were randomly divided into 4 groups (n =10 each) using a random number table,sham operation group (Group S),cardiopulmonary bypass group (Group C),Dex Ⅰ group (Group D Ⅰ),and Dex Ⅱ group (Group D Ⅱ).Rats in the Group G received no CPB procedure.CPB rat model was established in Group C,Group D Ⅰ and Group D Ⅱ.In Group D Ⅰ,Dex was infused at a rate of 2.5 μg/(kg · h) for 2 h during CPB after a loading dose of Dex by 2.5 μg/kg.In group D Ⅱ,Dex was infused at a rate of 5 μg/(kg · h) for 2 h during CPB after a loading dose of Dex by 5 μg/kg.Before CPB (T0),1 h after CPB (T1),and 2 h after CPB (T2),venous blood samples were collected for detection of plasma concentrations of interleukin 6 (IL-6),interleukin 10 (IL-10),S100β and NSE in using ELISA.Results Compared with Group S,concentrations of IL-6,IL-10,S100β and NSE at T1 and T2 were significantly increased in Group C (P ＜ 0.05).Compared with Group C,the concentrations of IL-6 and S100β at T2 were significantly reduced in Group D Ⅰ (P ＜0.05),as the concentrations of IL-6 and S100β at T1 and T2 were significantly decreased in Group D Ⅱ (P ＜ 0.05).Meanwhile,the concentrations of NSE at T1 and T2 were significantly reduced in Group D Ⅰ and Group D Ⅱ (P ＜0.05).Compared with Group D Ⅰ,the concentration of S100β at T1 was significantly reduced in Group D Ⅱ (P ＜ 0.05),and the concentration of NSE at T2 was significantly reduced in Group D Ⅱ (P ＜ 0.05).Conclusion Dex can attenuate the cerebral injury followed by CPB through inhibiting the inflammatory responses.And there is a certain correlation between the effect of cerebral protection and the doses of Dex.关键词
右美托咪定/体外循环/脑损伤/脑保护Key words
dexmedetomidine/cardiopulmonary bypass/cerebral injury/cerebral protection引用本文复制引用
陈燕桦,张炳东,韦祎,周丽芳,毛琦,谢玉波..右美托咪定对大鼠体外循环期间炎性因子、S100β和NSE的影响[J].广东医学,2017,38(11):1668-1670,3.基金项目
国家自然科学基金资助项目(编号:81373498),广西科学研究与技术开发计划项目(编号:桂科攻1355005-4-2),广西自然科学基金资助项目(编号:2015GXNSFBA139139),广西医科大学青年科学基金资助项目(编号:GXMUYSF2014041),广西高校中青年教师基础能力提升项目(编号:KY2016YB079) （编号:81373498）
