中国妇幼健康研究2017，Vol.28Issue(7)：747-752,757,7.DOI:10.3969/j.issn.1673-5293.2017.07.001
过敏性紫癜患儿肠道菌群结构及多样性研究
Structure and diversity of intestinal flora in children with Henoch-Schnlein purpura
摘要
Abstract
Objective To study the structure and diversity of intestinal flora and structure and diversity difference of fecal flora and mucosal flora in children with Henoch-Schnlein purpura (HSP).Methods Stool samples from 47 children with HSP were collected, including colon mucosa samples from 7 HSP children with abdominal symptoms, and stool samples from 11 healthy children were used as controls.DNA of samples was extracted using extraction kit method and subjected to high throughput sequencing, and biological information analysis was conducted on results.Results Structure of fecal flora was different between HSP children and healthy children.Predominant bacteria in two groups were Firmicutes (47.10%, 57.75%), Proteobacteria (23.04%, 10.13%) and Bacteroidetes (22.81%, 21.74%) at phylum, and Enterobacteriaceae (17.60%, 8.72%), Bacteroidaceae (18.90%, 21.65%), Ruminococcaceae (16.44%, 28.93%), Lachnospiraceae (11.55%, 10.74%) were more abundant at family.Abundances of Proteobacteria, Erwinia (from order to family), B-uniformis, Megamonas and Enterococcus (from family to genus) in fecal flora of HSP children were higher than those of children in the control group, while abundances of Streptococcus (from order to family), Bifidobacterium (from order to family) and F.prausnitzii (from family to species) in HSP children were lower than those in children in the control group.Diversity of fecal flora was higher than that of mucosal flora in HSP children with abdominal symptoms.Abundances of Firmicutes (53.64%), Proteobacteria (33.65%) and Bacteroidetes (9.24%) were higher in fecal flora at phylum, while abundance of Proteobacteria (88.13%) was higher in mucosal flora.Predominant bacteria in fecal flora were Enterobacteriales (27.18%), Ruminococcaceae (15.58%) and Lachnospiraceae (12.31%) at family, while predominant bacteria in mucosal flora was Enterobacteriaceae (87.19%).Bifidobacterium (from phylum to genus), Streptococcus (from phylum to genus), Planococcaceae (from phylum to family), Erysipelotrichaceae (from phylum to family), Clostridiaceae (from phylum to family), Ruminococcaceae (from phylum to family) and Lachnospiraceae (from phylum to family) were major in fecal flora, while Erwinia (from phylum to genus) was the major bacteria in mucosal flora.Conclusion Pathogenic bacteria or opportunistic pathogen are increased and beneficial bacteria are reduced in feces of children with HSP.Structure and diversity of fecal flora and mucosal flora are different in HSP children with abdominal symptoms.关键词
过敏性紫癜/肠道菌群/菌群结构/菌群多样性/高通量测序Key words
Henoch-Schnlein purpura (HSP)/intestinal flora/flora structure/flora diversity/high-throughput sequencing分类
医药卫生引用本文复制引用
陈鹏德,林燕,杨洁,郝丽军,雷达,兰莉,江逊,王宝西..过敏性紫癜患儿肠道菌群结构及多样性研究[J].中国妇幼健康研究,2017,28(7):747-752,757,7.基金项目
国家自然科学基金资助项目(编号:81370490) （编号:81370490）
