中国妇幼健康研究2017，Vol.28Issue(7)：793-796,831,5.DOI:10.3969/j.issn.1673-5293.2017.07.014
β-catenin、Smad3及TGF-β1在宫腔粘连患者子宫内膜组织中的表达及意义
Expressions and significance of β-catenin, Smad3 and TGF-β1 in endometrium of patients with intrauterine adhesion
摘要
Abstract
Objective To study the expression levels of β-catenin, Smad3 and TGF-β1 in adhesion tissue in patients with intrauterine adhesion (IUA) and to explore its mechanism in IUA.Methods A total of 60 cases of IUA treated with hysteroscopic lysis of intrauterine adhesions from May 2014 to May 2016 in Urumqi Maternal and Child Health Care Hospital were selected in experimental group, and 60 cases undergoing routine hysteroscopy were selected in control group.Real time fluorescent quantitative polymerase chain reaction (RT-PCR) was used to detect the expression levels of β-catenin mRNA, Smad3mRNA and TGF-β1 mRNA in adhesion tissue and endometrium near adhesion of IUA patients in experimental group and in normal endometrium in control group.Results Sequence of expression levels of β-catenin mRNA, Smad3 mRNA and TGF-β1 mRNA in normal endometrium in controls and in adhesion tissues in patients with mild, moderate and severe IUA was controls, mild IUA, moderate IUA and severe IUA patients from low to high, and difference had statistical significance (F value was 20.35, 19.62, and 22.32, respectively, all P<0.05).Expressions of β-catenin mRNA, Smad3 mRNA and TGF-β1 mRNA in adhesion tissues and endometrium near adhesions of IUA patients in experimental group were higher than those in normal endometrium in control group, and difference was statistically significant (P value was 0.038, 0.003 and 0.004, respectively).Expressions of β-catenin mRNA, Smad3 mRNA and TGF-β1 mRNA in adhesion tissue were higher than those in endometrium near adhesion, and difference was statistically significant (P value was 0.016, 0.001 and 0.008, respectively).Expression of TGF-β1 in adhesion tissue of IUA patients was positively correlated with that of Smad3 (rs=0.44, P=0.01).Expression of TGF-β1 was also positively correlated with that of β-catenin (rs=0.42, P=0.02), but expression of Smad3 had no correlation with that of β-catenin (rs=0.037,P=0.85).Conclusion High expressions of β-catenin, TGF-β1 and Smad3 in IUA patients may be closely related to the occurrence of IUA.Pathway of β-catenin and TGF-β1/Smad may be involved in endometrial fibrosis and promote the formation of IUA.关键词
宫腔粘连/β-连环蛋白/TGF-β1/Smad/纤维化Key words
intrauterine adhesion (IUA)/β-catenin/TGF-β1/Smad/fibrosis分类
医药卫生引用本文复制引用
路静,王玉平,张霆,郝友瑛..β-catenin、Smad3及TGF-β1在宫腔粘连患者子宫内膜组织中的表达及意义[J].中国妇幼健康研究,2017,28(7):793-796,831,5.基金项目
新疆维吾尔自治区自然科学基金资助项目(2015211C102) （2015211C102）
