国际口腔医学杂志2017，Vol.44Issue(4)：411-420,10.DOI:10.7518/gjkq.2017.04.009
luxS/AI-2密度感应对缓症链球菌生物膜致病力的影响
Biofilm formation and antimicrobial susceptibility of Streptococcus mitis in response to luxS/AI-2 signaling
摘要
Abstract
Objective This paper aims to study the influence of luxS gene mutation of Streptococcus mitis on biofilm formation and antimicrobial susceptibility.Methods Culture in blood plate,Gram stain and susceptibility tests,and physiological and biochemical tests were first carried out.16S ribosomal DNA(rDNA) sequence homology analysis of clinical isolate was examined subsequently.The autoinducer-2(AI-2) bioluminescence assay of Vibrio harveyi BB 170 strain,multidrug resistance,and ability of biofilm formation were examined in luxS mutant and clinical isolate.The structure,exopolysaccharides,and dead/live cells of biofilm were scanned by confocal laser scanning microscope.Compared with one another,luxS mutant growth recruited by isolate supernatant or 4,5-dihydroxy-2,3-pentanedione(DPD) was experimented.Results luxS gene deletion decreased biofilm formation(P＜0.001),loosened structure,and changed antimicrobial susceptibility(to ampiciliin,ciprofloxacin,tetracycline).However,isolate supernatant or DPD can recover the structure and ability of biofilm formation in luxS mutant.Conclusion luxS/AI-2 signaling can influence biofilm formation and antimicrobial susceptibility ofS.mitis with multidrug resistance.关键词
luxS基因/自体诱导物-2/密度感应/生物膜/抗菌药敏感性Key words
luxS gene/autoinducer-2/quorum sensing/biofilm/antimicrobial susceptibility分类
医药卫生引用本文复制引用
陆笑,翁春辉,王劲茗,刘少娟,刘芹,林珊..luxS/AI-2密度感应对缓症链球菌生物膜致病力的影响[J].国际口腔医学杂志,2017,44(4):411-420,10.基金项目
广州市科技计划应用基础研究专项项目(2011J4100036)This study was supported by Science and Technology Program of Guangzhou(2011J4100036). （2011J4100036）
