国际口腔医学杂志2017，Vol.44Issue(4)：421-425,5.DOI:10.7518/gjkq.2017.04.010
尼古丁和美卡拉明对牙周致病微生物的影响
Effect of nicotine and mecamylamine on growth of periodontal pathogens
摘要
Abstract
Objective This work aims to study the effects of nicotine and mecamylamine on periodontal pathogen growth and pathogen-formed biofilms.Methods Doubling dilution was used to determine the minimum inhibition concentration(MIC) and minimum bactericidal concentration(MBC) of nicotine on Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans.The influences of 1/16 MIC,1/8 MIC,1/4 MIC,1/2 MIC,and MIC of nicotine on the biofilms formed by P.gingivalis and A.actinomycetemcomitans were studied by light absorbance determination on a microplate reader.Results The MIC of nicotine against planktonic P.gingivalis was 8 mg·mL-1,which was similar to that of A.actinomycetemcomitans.When the nicotine concentration increased by a degree,the absorbance of the two bacterial biofilms increased.However,the absorbance decreased at further increase in nicotine concentration.The absorbance decreased to a minimum at a nicotine concentration of 8 mg·mL-1,which was also the minimum biofilm inhibition concentration.The group with mecamylamine(nicotine-receptor antagonist) showed higher absorbance than the control group.Conclusion Minimal doses of nicotine can promote the biofilm formation of P.gingivalis and A.actinomycetemcomitans,and continued increase in nicotine concentration inhibits biofilm formation.In the presence of mecamylamine,nicotine increases the biofilms formed by the P.gingivalis and A.actinomycetemcomitans.This effect is attributed to the decrease in nicotine concentration caused by mecamylamine.关键词
尼古丁/美卡拉明/牙龈卟啉单胞菌/伴放线放线杆菌/生物膜Key words
nicotine/mecamylamine/Porphyromonas gingivalis/Actinobacillus actinomycetemcomitans/biofilm分类
医药卫生引用本文复制引用
刘诗雨,田宓,石黎冉,潘韦霖,王一尧,李明云..尼古丁和美卡拉明对牙周致病微生物的影响[J].国际口腔医学杂志,2017,44(4):421-425,5.基金项目
国家自然科学基金(81400501) （81400501）
口腔疾病研究国家重点实验室专项经费(SKLOD201525) （SKLOD201525）
四川大学大学生创新训练计划(20140298)This study was supported by the National Natural Science Foundation of China(81400501),the Special Funds of State Key Laboratory of Oral Diseases(SKLOD201525) and Sichuan University Students' Innovative Training Program(20140298). （20140298）
