中国实用神经疾病杂志2017，Vol.20Issue(13)：1-4,4.
HOTAIR通过E-cadherin对胶质瘤侵袭和转移的影响研究
Study of HOTAIR effect invasion and metastasis of glioma by E-cadherin
摘要
Abstract
Objective To observe the effect of HOTAIR on invasion and metastasis of glioma.Methods Using q-PCR to test the relationship between HOTAIR and E-cadherin from clinical specimens.The invasion and metastasis of cells were tested through Wound Healing after HOTAIR overexperssion in the conventional culture SWO-38 and U251 cells in vitro.Western blotting was used to test the expression of C-cadherin protein in cells.Results In q-PCR glioma tissue,the expression average of E-cadherin was lower in HOTAIR high expression than that in HOTAIR low expression.The difference was statistically significant.After overexpression HOTAIR in SWO-38 and U251 cells,the expression E-cadherin was decreased through Western blotting detection.Wound Healing showed that the ability of metastasis was enhanced after HOTAIR overexpression.Conclusion HOTAIR can increase the invasion and metastasis of glioma,which may be related to the decreased E-cadherin expression.关键词
胶质瘤/HOTAIR/E-cadherin侵袭Key words
Glioma/HOTAIR/E-cadherin invasion分类
医药卫生引用本文复制引用
李锡清,赵尊兰,刘冬玲,刘明月,马宁,李明,周云..HOTAIR通过E-cadherin对胶质瘤侵袭和转移的影响研究[J].中国实用神经疾病杂志,2017,20(13):1-4,4.基金项目
国家自然科学基金(U1504820) （U1504820）
