解放军医学杂志2017，Vol.42Issue(8)：663-667,5.DOI:10.11855/j.issn.0577-7402.2017.08.01
巨噬细胞胆碱能通路对急性缺糖缺氧性肾小管细胞损伤的影响
The cholinergic pathway alleviates acute oxygen and glucose deprivation induced renal tubular cell injury by reducing the secretion of inflammatory medium of macrophages
摘要
Abstract
Objective To investigate the effects of cholinergic pathway on acute renal tubular cell injury induced by acute oxygen and glucose deprivation. Methods Rat kidney macrophages were isolated and cultured for constructing macrophages and renal epithelial cells co-cultivating model of oxygen-glucose deprivation (OGD), and the model cells were divided into three groups: OGD alone group, acetylcholine (ACh 100μmol/L)+OGD group and ACh + galantamine (Gal 10μmol/L)+OGD group. The cells underwent OGD treatment for 1 hour, and normally cultured for 24 hours. The expressions of TNF alpha, IL-1 beta, and IL-10 in supernatant fluid were detected by ELISA, the renal tubular cell viability was determined by MTT assay, the expression of acetylcholine esterase (AChE) mRNA and protein were determined by RT-qPCR and Western blotting. The activity of AChE was determined by colorimetric method. Results The expressions of TNF alpha (pg/ml) in OGD, Ach+OGD group, Ach+Gal+OGD groups were 140.2±44.81, 119.46±4.42 and 103.31±1.62 respectively (P<0.05), those of IL-1β (pg/ml) were 172.26±13.51,144.34±5.53 and 119.37±11.42 respectively (P<0.05), and those of IL-10 (pg/ml) were 181.47±16.01, 173.62±10.12 and 188.36±8.73 respectively (P>0.05); The values of renal tubular cell proliferation were 55.02%±6.28%, 66.65%±6.47%, and 79.75%±4.22% respectively (P<0.01); the expressions of AChE mRNA in macrophages were 4.07±0.03, 4.22±0.15 and 3.98±0.29 respectively in the three groups (P>0.05); those of AchE protein were 0.66±0.07, 0.74±0.04 and 0.67±0.06 respectively (P>0.05); The activity of AChE (kU/L) was 0.51±0.02, 0.35±0.05 and 0.32±0.04 respectively (P=0.001, 0.001 and 0.368). Conclusions ACh and Gal could inhibit the secretion of inflammatory mediators and cholinesterase activity and can reduce the acute hypoxic renal tubular cell injury. The modulation of the cholinergic pathway in macrophages may be the important treatment method for acute renal injury in the future.关键词
胆碱能药/炎症介导素类/肾小管Key words
cholinergic agents/inflammation mediators/acute kidney injury分类
医药卫生引用本文复制引用
吴明,吴乐锋,陆俊福,李明利,李赟,徐迹,刘文兰,刘芬,冯永文..巨噬细胞胆碱能通路对急性缺糖缺氧性肾小管细胞损伤的影响[J].解放军医学杂志,2017,42(8):663-667,5.基金项目
国家自然科学基金(81101410) （81101410）
广东省医学科学技术研究基金(A2016353) （A2016353）
广东省深圳市科技创新委项目(JCYJ20130401112313541、JCY20150330102401099、JCYJ20160425103130218) （JCYJ20130401112313541、JCY20150330102401099、JCYJ20160425103130218）
深圳市第二人民医院临床-基础一对一桥梁项目基金(2015-16)This work was supported by the National Natural Science Foundation of China (81101410), the Medical Science and Technology Research Foundation of Guangdong (A2016353), Shenzhen Science and Technology Planning Project (JCYJ20130401112313541, JCYJ20150330102401099, JCYJ20160425103130218), and Clinical Doctor-Basic Scientist Combination Foundation of Shenzhen Second People's Hospital (2015-16) （2015-16）
