解放军医学杂志2017，Vol.42Issue(8)：668-673,6.DOI:10.11855/j.issn.0577-7402.2017.08.02
饱和脂肪酸和不饱和脂肪酸对肺癌细胞增殖与自噬的影响
Effects of saturated and unsaturated fatty acids on proliferation and autophagy of lung cancer cells
摘要
Abstract
Objective To investigate the effects of saturated fatty acids and unsaturated fatty acids on proliferation and autophagy of human lung cancer cells. Methods The lung cancer cells A549 were treated with stearic acid (saturated fatty acid) and doconexent (DHA, unsaturated fatty acid), respectively, in concentrations of 0, 30, 60, 120 and 240μmol/L. MTT test and cell clone formation assay were performed to detect the proliferation of A549 cells. The morphology of A549 autophagy was observed by confocal laser scanning microscopy after A549 cells were treated with stearic acid or DHA for 24 hours. Western blotting assay was used to detect the expression of autophagy-related protein after A549 cells were treated with stearic acid or DHA for 12, 24 and 36 hours, respectively. Results 30-240μmol/L stearic acid or DHA both inhibited the proliferation of A549 cells (P<0.05). Both stearic acid and DHA induced autophagy of A549 cells, meanwhile, down-regulated Phospho-mTOR (ser2481) and up-regulated LC3Ⅱ/LC3Ⅰ of A549 cells (P<0.05). Conclusions Both saturated fatty acid and unsaturated fatty acid can inhibit the proliferation and induce autophagy of lung cancer cells. The mechanisms of autophagy may be related to Phospho-mTOR (ser2481) signaling pathway.关键词
硬脂酸类/二十二碳六烯酸类/A549细胞/细胞增殖/自噬/哺乳动物雷帕霉素靶蛋白Key words
stearic acids/docosahexaenoic acids/A549 cells/cell proliferation/autophagy/mammalian target of rapamycin分类
医药卫生引用本文复制引用
李慧敏,伍俊,吴尚,余华军,汪亚君,熊禹真,兰柳波,张海涛..饱和脂肪酸和不饱和脂肪酸对肺癌细胞增殖与自噬的影响[J].解放军医学杂志,2017,42(8):668-673,6.基金项目
国家自然科学基金(81273549) （81273549）
广东省科技计划项目(2014A020212292)This work was supported by the National Natural Science Foundation of China (81273549), and the Guangdong Science and Technology Project (2014A020212292) （2014A020212292）
