解放军医学杂志2017，Vol.42Issue(8)：692-697,6.DOI:10.11855/j.issn.0577-7402.2017.08.06
应激刺激对大鼠心肌细胞快激活延迟整流钾电流改变的影响
Effects of stress on the rapid component of delayed rectifier potassium current in rat cardiomyocytes
摘要
Abstract
Objective To observe the effect of stress on the rapid component of delayed rectifier potassium current (IKr) in rat cardiomyocytes. Methods Forty male SD rats were randomly divided into four groups (10 each): control group (Ctrl), exhaustive group (ES), noise group (WN) and composite group (ES+WN). Stress animal models were prepared as follows: Rats in ES group were undergoing exhaustive swimming as the stress factor, in WN group undergoing white noise and in ES+WN group undergoing exhaustive swimming + white noise as the stress factor. Langendorff device was used to reversely perfuse collagenase for isolating the rat's ventricular myocytes. The effect of stress on IKr current and gating mechanism of single ventricular myocyte was recorded by whole-cell patch clamp technique. Results Compared with the Ctrl group, the tail current density of IKr (IKr,tail) of ventricular myocytes increased significantly in ES group and WN group (P<0.01). The IKr,tail current density of the ventricular myocytes in ES+WN group was significantly higher than that in ES group and WN group (P<0.01), and the effect was voltage dependent. Gating mechanism revealed that the half inactivation voltage of IKr,tail (V1/2,inact) can be shifted to the right in ES group, WN group and ES+WN group when compared with the Ctrl group, and the recovery time constant shortened after inactivation (P<0.01). However, the steady-state activation, fast inactivation constant and voltage dependence of IKr,tail were not statistically significant in ES group, WN group and ES+WN group when compared with the Ctrl group. Conclusion Stress increases the IKr current in rat cardiomyocytes, suggesting it be one of the electrophysiological bases of stress-induced arrhythmia.关键词
应激刺激/心律失常/快激活延迟整流钾电流/膜片钳技术Key words
stress/arrhythmia/IKr current/patch clamp technique分类
医药卫生引用本文复制引用
齐江莉,蔡钟奇,董颖,陈韵岱,李泱,解亚楠,李旺,曹雪滨..应激刺激对大鼠心肌细胞快激活延迟整流钾电流改变的影响[J].解放军医学杂志,2017,42(8):692-697,6.基金项目
国家自然科学基金(81170177,81470542,81570349) （81170177,81470542,81570349）
全军医学科技"十二五"科研项目(CBJ13J002)This work was supported by the National Natural Science Foundation of China (81170177, 81470542, 81570349) and the Medical Sciences "Twenfth Five-Year Plan" Research Project of Chinese PLA (CBJ13J002) （CBJ13J002）
