解放军医学杂志2017，Vol.42Issue(8)：698-701,4.DOI:10.11855/j.issn.0577-7402.2017.08.07
体外光化学疗法处理的调节性树突细胞对T细胞增殖的影响
Influence of extracorporeal photochemotherapy-treated regulatory dendritic cells on T cell proliferation
摘要
Abstract
Objective To investigate the effect of regulatory dendritic cells treated by extracorporeal photochemotherapy on T cell proliferation. Methods Human peripheral blood mononuclear cells (PBMCs) were obtained and the immature dendritic cells (imDCs) were induced by recombinant human granulocyte and macrophage colony stimulating factors. SPDCs were obtained by PUVA treatment, and ECDCs were co-cultured with imDCs and PUVA-SP to obtain immunoprecipitated dendritic cells. In vitro, imDCs were co-cultured with SPDCs to obtain SPDCs; imDCs were added to 10ng/ml of LPS, and cultured for 1 day to obtain DCs. The expressions of CD11c, CD83 and CD86 on the surface of the cells were detected. The effect of imDCs on the proliferation of recipient T cells was detected by mixed lymphocyte culture method. Results The early apoptosis rate of PUVA-treated cells was 91.33%. The positive expression rates of CD83 and CD86 in ecpDCs were 22.83%±5.26% and 22.06%±4.37%, respectively, which were similar to those of imDCs (15.06%±0.59%, 15.19%±1.83% (P<0.01), but significantly lower than those in DCs (99.79%±0.36%, 99.85%±0.19%, respectively), the difference was statistically significant (P<0.01). The recipient imDC cells phagocyting the appoptotic splenic lymphocytes from the donor significantly inhibited the proliferation of recipient T cells. Conclusion Apoptosis of splenic lymphocytes induced by extracorporeal photochemotherapy can inhibit the maturation of dendritic cells and inhibit the proliferation of T lymphocytes.关键词
体外光化学疗法/树突细胞/移植免疫Key words
extracorporeal photochemotherapy/dendritic cells/transplantation immunity分类
医药卫生引用本文复制引用
杜国盛,杨绍臻,魏玉香,索龙龙,封立魁,宋继勇,朱志东..体外光化学疗法处理的调节性树突细胞对T细胞增殖的影响[J].解放军医学杂志,2017,42(8):698-701,4.基金项目
国家自然科学基金(811022422)This work was supported by the National Natural Science Foundation of China (811022422) （811022422）
