解放军医学杂志2017，Vol.42Issue(8)：702-706,5.DOI:10.11855/j.issn.0577-7402.2017.08.08
迷走神经电刺激对大脑中动脉阻断/再灌注大鼠脑损伤及脑组织中p-CREB表达的影响
Effects of vagus nerve stimulation on cerebral ischemia injury and expression of p-CREB in rat MCAO/reperfusion models
摘要
Abstract
Objective To investigate the effects of vagus nerve electrostimulation (VNS) on the brain damage of rat middle cerebral artery occlusion (MCAO)/reperfusion model and its mechanism. Methods Twenty four adult male SD rats were randomly divided into two groups (12 each): MCAO/reperfusion group (MCAO group) and MCAO/reperfusion+VNS group (MCAO+VNS group). Subsequently, the neurological function deficit was determined by neurological scoring according to Zea Long scoring method 24h after MCAO/reperfusion. The cerebral infarction volume was determined by TTC assay. The cell apoptosis in brain damage zone was determined by TUNEL assay. Then, the effect of VNS on cAMP response element binding protein (CREB) and p-CREB protein expression was determined by Western blotting. The effect of VNS on Bcl-2 and Bax expression was determined by immunohistochemistry assay. Results Compared with MCAO group, VNS significantly inhibited MCAO-induced neurological deficit (P<0.01), decreased brain infarct volume (P<0.01) and cell apoptosis (P<0.01), increased the expression of p-CREB protein (P<0.01) and the number of Bcl-2-position cells (P<0.01) together with decreasing the number of Bax-position cells (P<0.01). However, VNS did not affect the expression of CREB protein (P>0.05). Conclusion VNS may ameliorate MCAO-induced neurological deficit and decrease brain infarct volume, which may be related to the promotion of p-CREB protein expression level.关键词
迷走神经电刺激/cAMP反应元件结合蛋白/大脑中动脉阻断/细胞凋亡Key words
vagus nerve stimulation/cAMP response element binding protein/middle cerebral artery occlusion/apoptosis分类
医药卫生引用本文复制引用
郭佳,关智媛,孙守元,金晶,李鸣明,卜玉洁,张廷华..迷走神经电刺激对大脑中动脉阻断/再灌注大鼠脑损伤及脑组织中p-CREB表达的影响[J].解放军医学杂志,2017,42(8):702-706,5.基金项目
2015年度兰州大学第二医院院内博士科研基金(YNBSKYJJ2015-1-29) （YNBSKYJJ2015-1-29）
甘肃省青年科技基金计划项目(1506RJYA246)This work was supported by the Doctoral research fund of the Second Hospital of Lanzhou University in 2015 (YNBSKYJJ2015-1-29) and the Gansu Provincial Youth Science and Technology Fund (1506RJYA246) （1506RJYA246）
