军事医学2017，Vol.41Issue(6)：419-423,429,6.DOI:10.7644/j.issn.1674-9960.2017.06.003
IL-21通过 SENP1调控 NK细胞生物学特性
IL-21 modulates biological characteristics of NK92 cells by upregulating SENP1 expression
摘要
Abstract
Objective To investigate the roles of SENP1 in regulation of biological characteristics of NK cells.Methods Lentivirus-mediated-Senp1-small-hairpinRNA (shRNA) transduction was applied to NK92 cells.The expression of SENP1 in NK92 cells was determined by real-time PCR and Western blot.The proliferation of NK92 cells was detected by CCK-8 assay.The apoptosis of NK92 cells was determined by Annexin Ⅴ and PI labeling.The cytotoxicity of NK92 cells against K562 cells was evaluated by luciferase reporter assay.Results Treatment of NK92 cells with IL-21 resulted in SENP1 upregulation.Lentivirus mediated SENP1 knockdown reduced proliferation and increased apoptosis in NK-92 cells,but SENP1 inhibition had slight impact on the cytotoxic ability of NK92 cells to kill K562 cells.Conclusion SENP1 mediates the regulatory effect of IL-21 on the proliferation and survival of NK92 cells.关键词
受体,白细胞介素21/小泛素相关修饰蛋白质类/杀伤细胞,天然/细胞增殖/细胞凋亡Key words
receptors/interleukin-21/small ubiquitin-related modifier proteins/killer cells/natural/cell proliferation/apoptosis分类
生物科学引用本文复制引用
李宇翔,徐芹芹,孙慧燕,魏静,张晓艳,贾庆华,肖凤君,王立生..IL-21通过 SENP1调控 NK细胞生物学特性[J].军事医学,2017,41(6):419-423,429,6.基金项目
国家自然科学基金资助项目(81470320) （81470320）
