军事医学2017，Vol.41Issue(6)：430-433,439,5.DOI:10.7644/j.issn.1674-9960.2017.06.005
过表达细胞间黏附分子1的间充质干细胞治疗小鼠1型糖尿病性肾病的实验研究
Therapeutical effect of mesenchymal stem cells overexpressing ICAM-1 in murine type 1 diabetes nephropathy
摘要
Abstract
Objective To explore the curative effects of mesenchymal stem cells(MSC)that overexpress in murine type 1 diabetes nephropathy (DN).Methods Mice were randomly divided into normal control(NC) group,DN group,C3-treated group,C3-MIGR1-treated group and C3-MIGR1-ICAM-1-treated group.Mice were given streptozotocin until the DN model was set up.The murine DN model was treated with murine MSC(C3H10T1/2),transfection empty vector of murine MSCs(C3H10T1/2-MIGR1/MSC) and murine MSCs (C3H10T1/2-ICAM-1/MSC)that overexpressed ICAM-1.After transplantation, the pathological features of kidneys were observed by Masson staining and the number of homing MSC cells to the kidney was calculated on days 1,3,7 by frozen section, while qPCR was used to analyze the expression of signaling molecules for collagen1, TGF-β1 and SMAD2 after treatment with various MSCs.Results Compared with DN group, the renal fibrosis treated with MSCs overexpressing ICAM-1 was significantly decreased by Masson staining.Three and seven days after transplant, the homing cells of MSC in different groups displayed no difference using tissue freezing section method.Furthermore, TGF-β1/SMAD signaling was lowly activated after the treatment with MSCs that overexpressed ICAM-1 compared with model mice(P<0.01).Conclusion MSCs that overexpress ICAM-1 can protect kidneys in the DN model.关键词
细胞间黏附分子1/间质干细胞/糖尿病肾病/链脲菌素Key words
intercellular adhesion molecule-1/mesenchymal stem cells/diabetic nephropathies/streptozocin分类
医药卫生引用本文复制引用
张彦彦,李雪,马士凤,白博乾,刘元林,郑荣秀,张毅..过表达细胞间黏附分子1的间充质干细胞治疗小鼠1型糖尿病性肾病的实验研究[J].军事医学,2017,41(6):430-433,439,5.基金项目
国家自然科学基金资助项目(31070996,31171084) （31070996,31171084）
国家重点研发计划资助项目(2016YFC1000305) （2016YFC1000305）
原能细胞集团科技有限公司基金资助 （）
天津市卫生计生委科技基金攻关项目(16KG123) （16KG123）
天津市卫生局重点资助项目(17JCZDJC36400) （17JCZDJC36400）
