军事医学2017，Vol.41Issue(6)：434-439,6.DOI:10.7644/j.issn.1674-9960.2017.06.006
人脐带间充质干细胞来源的外泌体免疫调节功能异质性研究
Heterogeneity of immunomodulatory function of exosomes derived from human umbilical cord mesenchymal stem cells
摘要
Abstract
Objective To investigate the heterogeneity of immunomodulatory function of exosomes secreted from human umbilical cord-derived mesenchymal stem cells(hUC-MSC).Methods Five different hUC-MSCs lines were isolated and cultured.Exosomes were isolated from the supernatant.The expression of specific surface markers CD9,CD63,CD81 and CD44 was detected by flow cytometry.The protein concentration of hUC-MSCs exosomes(hUC-MSCs-ex)was evaluated by the BCA assay.Concanavalin A and rhIL-2 stimulated umbilical cord blood mononuclear cells (UBMCs) from healthy donor were co-cultured at different concentrations of hUC-MSCs-ex from different cell lines for 72 h.The growth rate of UBMCs was detected by MTT assay.ELISA was used to test the levels of IFN-γ and TNF-α of the supernatants.The UBMCs co-cultured with hUC-MSCs were co-cultured with K562 cells at the ratio of 5∶1.The cytotoxic activity was calculated by MTT assay.Results hUC-MSCs-ex expressed CD9,CD63,CD81and CD44.Different hUC-MSCs lines had different regulating activities on the proliferation,secretion and killing ability of UBMCs.Conclusion hUC-MSCs-ex has heterogeneity of immunomodulatory function on UBMCs.关键词
外泌体/间充质干细胞/脐带血单个核细胞/免疫调节/肿瘤坏死因子αKey words
exosomes/mesenchymal stem cells/umbilical cord blood mononuclear cells/immune regulation/tumor necrosis factor-alpha分类
医药卫生引用本文复制引用
梁亚会,张青宜,郭子宽,王芳,王恒湘..人脐带间充质干细胞来源的外泌体免疫调节功能异质性研究[J].军事医学,2017,41(6):434-439,6.基金项目
首都卫生发展科研专项资助项目(2014-2-5122) （2014-2-5122）
