军事医学2017，Vol.41Issue(6)：449-452,4.DOI:10.7644/j.issn.1674-9960.2017.06.009
聚合酶螺旋反应快速检测甲型H1N1流感病毒
Rapid detection of influenza A/H1N1 virus by polymerase spiral reaction
摘要
Abstract
Objective To establish a polymerase spiral reaction (PSR) method for rapid detection of influenza A/H1N1 virus.Methods Six sets of primers were designed for influenza A/H1N1 virus HA gene, and the results were determined with real time kinetic turbidimetric assay and colorimetry method.Results and Conclusion The best primers were selected from six sets of primers, and the best temperature was determined as 65 degrees Celsius.Further experiments showed that the best primer had good specificity for detection of influenza A/H1N1 virus,without cross reactions with 14 other respiratory tract pathogenic nucleic acids.The sensitivity was up to 100 copies,and consistent with that of PCR.So a PSR method is established for rapid detection of the influenza A/H1N1 virus, which is simple, quick, highly specific and sensitive,and especially applicable to field and grass-roots units.关键词
聚合酶螺旋反应/核酸恒温扩增/甲型H1N1流感病毒/快速检测Key words
polymerase spiral reaction/nucleic acid isothermal amplification/influenza A virus/H1N1 subtype/rapid detection分类
医药卫生引用本文复制引用
马文,刘威,黄留玉,董德荣,邹大阳,刘宁伟,贺晓明,敖大,杨展,黄思妺,徐雅晴..聚合酶螺旋反应快速检测甲型H1N1流感病毒[J].军事医学,2017,41(6):449-452,4.基金项目
国家自然科学基金资助项目(81501836) （81501836）
