军事医学2017，Vol.41Issue(6)：453-456,4.DOI:10.7644/j.issn.1674-9960.2017.06.010
高原地区55型腺病毒的病原分离及全基因序列分析
Pathogen isolation and whole-genome sequence analysis of human adenovirus type 55 outbreak on plateaus
摘要
Abstract
Objective To investigate the variation characteristics of adenovirus type 55 (HAdV-B55) gene on plateaus.Methods Throat swabs were collected from HAdV-B55 infected patients and used for virus isolation in HEp-2 cells.The whole-genome sequence was obtained by PCR and sequencing.HAdV-B55 gene sequence was blast with the previously reported virus.Results HAdV-B55 strains were isolated from throat swabs, which were named LS89/Tibet/2016.The whole-genome sequence was obtained and submitted to GenBank with the accession number of KY002683.No large fragment gene recombination was found between this HAdV-B55 strain and previous strains, and the sequence similarity with QS-DLL strain was 99.9%.Conclusion This study provides more information for the evolution patterns of adenovirus 55 and will contribute to the prevention and control of HAdV-B55 infection in the future.关键词
高原/腺病毒/HAdV-B55/病原分离/全基因测序Key words
plateau/adenovirus/HAdV-B55/pathogen isolation/whole-genome sequencing分类
医药卫生引用本文复制引用
王文博,郭平,邱薇,胡小兵,范泉水,刘媛,周奕帆,古良琪,张雪莲,张林,陈锚锚,杨显君,邹自英..高原地区55型腺病毒的病原分离及全基因序列分析[J].军事医学,2017,41(6):453-456,4.基金项目
全军后勤"十二五"重大专项资助项目(AWS11L009) （AWS11L009）
军队"十二五"重点课题资助项目(BWS14J025) （BWS14J025）
国家自然科学基金资助项目(81301445) （81301445）
全军医学科技青年培育项目(14QNP057) （14QNP057）
上海市医学生物防护重点实验室开放课题资助项目(SKLM1401) （SKLM1401）
