军事医学2017，Vol.41Issue(6)：498-501,4.DOI:10.7644/j.issn.1674-9960.2017.06.020
氢气通过诱导血红素加氧酶1表达减轻氧中毒急性肺损伤的实验研究
Hydrogen reduces hyperoxic acute lung injury by inducing heme oxygenase 1 expression
摘要
Abstract
Objective To investigate the mechanism by which hydrogen(H2) helps prevent acute lung injury induced by hyperoxia (HALI) in rats.Methods Thirty male Sprague-Dawley rats were randomly divided into three groups: control group, HALI group and H2 group, with 10 rats in each group.The control group was exposed to air at atmospheric pressure.Rats in HALI and H2 groups were exposed continuously to pure oxygen (100%O2) for 60 hours and during this period, 10 ml/kg of normal saline or H2-saturated normal saline was given every 12 hours by intraperitoneal injection to the HALI and H2 groups, respectively.After treatment, the arterial partial pressure of oxygen was examined and histopathological examination was conducted in each group.Then,RT-qPCR and Western blotting were performed to measure the transcriptional level and protein expression of heme oxygenase 1 (human heme oxygenase 1, HO-1) in rat lung tissue.Results Compared with the HALI group, H2 group showed significantly decreased severity of lung injury and a marked increase in the arterial oxygen saturation.Besides, H2 treatment induced up-regulation of HO-1 mRNA and protein levels.Conclusion The findings suggest that HO-1 may play an important role in the protection against HALI by H2.关键词
氢/氧/中毒/急性肺损伤/血红素加氧酶1Key words
hydrogen/oxygen/poisoning/acute lung injury/heme oxygenase 1分类
医药卫生引用本文复制引用
韩文杰,马玉媛,贾俊婷,欧敏,章金刚..氢气通过诱导血红素加氧酶1表达减轻氧中毒急性肺损伤的实验研究[J].军事医学,2017,41(6):498-501,4.基金项目
海军总医院创新培育基金资助项目(CXPY201409) （CXPY201409）
