南京农业大学学报2017，Vol.40Issue(4)：573-583,11.DOI:10.7685/jnau.201612014
水稻矮秆突变体htr的表型分析及基因克隆
Phenotypic analysis and gene cloning of a dwarf mutant htr in rice
摘要
Abstract
[Objectives]The aim is to identify rice dwarf mutant htr,localize the target gene and provide new dwarf gene resources for rice breeding.[Methods]Sections of the stem were prepared for cytological observation.The dwarf mutant htr was crossed with ‘ N22'and‘ 9311'to construct F2 populations for genetic analysis and gene mapping,respectively.We selected the recessive extreme individuals to localize the gene HTR.[Results]htr was a stably inherited dwarf mutant.The plant height,panicle length,1 000-grain weight,and tiller number in mature plants all decreased compared with wild type,while the leaf width and culm diameter were increased and the leaf colour of htr was much darker green than wild type.By observing sections of the second internodes at the mature stage,we found in the longitudinal direction of the stem,the cells failed to elongate normally and became small,but the cell number increased.Genetic analysis showed that the dwarf trait of mutant htr was controlled by a pair of recessive gene.The HTR locus was mapped to a region between the markers ttr-1 and ttr-2 on chromosome 5,which was further narrowed down to a 144 kb region.Sequence analysis revealed that the coding region of the AP2-like ethylene-responsive transcription factor had a single nucleotide substitution,resulting in a change in the conserved amino acid sequence in the htr mutant.Subcellular localization assay showed that HTR was located in the nucleus,and the HTR was constitutively expressed,but it had the highest expression in the stem.[Conclusions]The phenotype of htr was controlled by a single recessive gene,which encodes the AP2-like ethylene-responsive transcription factor.The cloning of HTR gene could provide some theoretical reference for cultivating thick-walled and lodging resistant new rice varieties.关键词
水稻/矮秆/基因克隆/转录因子/AP2Key words
rice (Oryza sativa L.)/dwarf/gene cloning/transcription factor/AP2分类
农业科技引用本文复制引用
董邦宁,龙武华,刘喜,刘世家,田云录,陈亮明,江玲,王益华,万建民..水稻矮秆突变体htr的表型分析及基因克隆[J].南京农业大学学报,2017,40(4):573-583,11.基金项目
国家重点研发项目七大农作物育种专项(2016YFD0100101-08) （2016YFD0100101-08）
国家863计划项目(2014AA10A603-15) （2014AA10A603-15）
江苏省科技支撑计划项目(BE2014394,BE2015363) （BE2014394,BE2015363）
江苏省农业科技自主创新资金课题(CX(16)1029) （CX(16）
