南京农业大学学报2017，Vol.40Issue(4)：664-670,7.DOI:10.7685/jnau.201606030
高效溶磷菌的筛选及其促生效应研究
Screening phosphate solubilizing bacterial strains from maize rhizosphere and research on their plant growth promotion effect
摘要
Abstract
[Objectives]This study aimed to isolate phosphate-solubilizing bacterial strains from maize rhizosphere first,then to obtain one strain with best plant growth promotion ability from them though pot experiments and to evaluate its crop yield promotion effect using field experiments.[Methods]Modified PVK medium was used to screen phosphate-solubilizing strains qualitatively,then NBRIP liquid shake flask experiments were performed to evaluate the phosphate solubilizing ability of isolated strains,after that growth promotion and crop yield increasing effects of the strains were determined through pot and field experiments.[Results]From the rhizosphere soil of maize,eight bacterial strains with inorganic phosphorus-solubilizing ability were selected.487.67 mg· L-1 of soluble phosphorus content was observed in NBRIP liquid culture medium inoculated with a strain named NJAU-B8 which showed higher ratio of transparent circle diameter to cell diameter.Pot experiment results showed a higher growth promoting effect of maize seedling was observed after inoculating strain NJAU-B8 than other strains and control.Compared to the control without inoculation,plant height,stem diameter,chlorophyll,fresh weight and dry weight of shoot were increased by 40.37％,18.74％,1.32％,46.67％ and 4.08％,respectively,in the first season pot experiment and by 22.18％,2.02％,1.20％,16.26％ and 35.53％ in the second season pot experiment.Moreover,inoculation of other strains also showed higher plant height than the control except strain B1.No significant difference in the crop yield of maize was observed among different treatments in the first season field experiment,nevertheless,the yield in compound microbial fertilizer (BIO)treatment was higher compared to chemical fertilizer(CF) and organic-inorganic compound fertilizer(AOF) treatments;in the second field experiment,cabbage yield in BIO treatment was significantly higher than that in AOF and CF treatments;and in the third field experiment,potato yield in BIO treatment increased by 11.29％ and 26.41％ compared to AOF and CF,respectively.Combined with the morphological,physiological and biochemical characteristics and 16S rDNA gene sequence analysis,strain NJAU-B8 was classified into genus Burkholderia.[Conclusions]The isolated phosphate solubilizing bacterial strain NJAU-B8 can promote the growth of maize in the pots and increase crop yield in the field effectively.关键词
溶磷菌/溶磷量/玉米/促生/增产Key words
phosphate solubilizing bacteria/phosphate solubilizing amount/maize/plant growth promotion/crop yield increase分类
农业科技引用本文复制引用
乔策策,王甜甜,王若斐,刘超,高琦,李荣,沈其荣..高效溶磷菌的筛选及其促生效应研究[J].南京农业大学学报,2017,40(4):664-670,7.基金项目
国家863计划项目(2013AA102802) （2013AA102802）
中央高校基本科研业务费专项资金项目(KYZ201519) （KYZ201519）
国家重点研发专项(2016YFD0800605,2016YFD0200106) （2016YFD0800605,2016YFD0200106）
江苏省农业科技自主创新资金项目[CX(15)100606] （15）
苏北专项(BN2015082) （BN2015082）
青蓝工程资助项目 （）
南京农业大学SRT项目(1513A09) （1513A09）
江苏省高校品牌专业建设工程资助项目(PPZY2015A061) （PPZY2015A061）
