南京农业大学学报2017，Vol.40Issue(4)：739-743,5.DOI:10.7685/jnau.201612009
动物过敏原牛血清白蛋白表面等离子共振传感器检测方法的建立
Development of a surface plasmon resonance sensor for detection of animal allergen bovine serum albumin
摘要
Abstract
[Objectives]A rapid,accurate and sensitive method for the quantitative detection of animal allergen bovine serum albumin (BSA) was developed using surface plasmon resonance (SPR)technology.[Methods]SPR was developed by monitoring the interaction between BSA and a self-prepared mouse anti-BSA monoclonal antibody on the SPR sensor chip,then determined for limit of detection (LOD)and optimal linear range,verified for reproducibility and applied preliminarily.[Results]The specific monoclonal antibody against BSA at a labeling level of 18 000 showed good affinity to BSA.The BSA concentration at a range of 6.25-400 ng· mL-1 showed good linear relationship to the response value of SPR analyzer(R2=0.992),with LOD of 33.68 ng· mL-1.The RSD of intra-and inter-assays on BSA at various concentrations by the developed SPR method was 1.12％-3.42％ and 0.84％-3.98％,respectively,both of which were less than 5％.No significant differences were observed between the determination results of BSA of commercial meat and meat products by SPR and ELISA methods (P＞0.05).[Conclusions]The SPR method constructed in the paper has high reliability and stability,and can be used for high-throughput and real-time detection of animal allergen BSA in food.关键词
牛血清白蛋白/动物过敏原/表面等离子共振/检测Key words
bovine serum albumin/animal allergen/surface plasmon resonance(SPR)/detection分类
轻工纺织引用本文复制引用
王玮,滕爽,朱业培,徐幸莲,周光宏..动物过敏原牛血清白蛋白表面等离子共振传感器检测方法的建立[J].南京农业大学学报,2017,40(4):739-743,5.基金项目
国家自然科学基金项目(31501395) （31501395）
江苏省自然科学基金项目(BK20140722) （BK20140722）
