山东农业科学2017，Vol.49Issue(5)：124-128,5.DOI:10.14083/j.issn.1001-4942.2017.05.027
利用硫氰酸锂水溶液提取云南琥珀蚕茧壳丝蛋白的适宜条件研究
Suitable Extraction Conditions for Yunnan Antheraea assama Silk Protein using Aqueous Solution of Lithium Thiocyanate
摘要
Abstract
Antheraea assama is an important economic insect.Its silk shows excellent features,and has a good potential for development and utilization in the fields of textile,biological medicine,biological material and so on.This research used lithium thiocyanate solution to extract A.assama silk protein.The results showed that some A.assama silk protein could be extracted and the extraction efficiency increased with the increase of temperature.At the set maximum bath temperature of 60℃,the protein concentration was 10.257 mg/mL after dissolved for 12 hours and the extraction rate was 8.91％.When keeping the solution temperature of 60℃ and proceeding ultrasonic treatment (53 kHZ,500 W) for 12 hours,the protein concentration reached 14.552 mg/mL and the extraction rate was 16.93％.SDS-PAGE gel electrophoresis detection results showed that there was no significant difference in the bands of silk protein extracted under different conditions,and there was no obvious degradation of silk protein.The extracted silk protein in both the concentration and quality could meet the needs of laboratory research.关键词
琥珀蚕/茧壳/丝蛋白/硫氰酸锂Key words
Antheraea assama/Cocoon/Silk protein/Lithium thiocyanate分类
农业科技引用本文复制引用
李琼艳,刘增虎,杨伟克,钟健..利用硫氰酸锂水溶液提取云南琥珀蚕茧壳丝蛋白的适宜条件研究[J].山东农业科学,2017,49(5):124-128,5.基金项目
云南省农业科学院蚕桑蜜蜂研究所青年创新基金项目(QC2015003) （QC2015003）
