山东医药2017，Vol.57Issue(22)：12-14,3.DOI:10.3969/j.issn.1002-266X.2017.22.004
玉屏风散对低温环境下小鼠肺脏固有淋巴样细胞比例及功能的影响
Influence of Yupingfeng powder on proportion and function of lung-resident innate lymphoid cells in mice under hypothermia
摘要
Abstract
Objective To observe the influence of Yupingfeng powder on the proportion and function of lung-resident innate lymphoid cell subsets (ILCs) in mice under hypothermia.Methods Twenty-four C57BL/6 mice were randomly divided into three groups:the control group,the hypothermia model group,and the group of Yupingfeng powder (YPF group).The mice in the control group were not given any treatment.The mice from hypothermia model group and YPF group were both exposed to a cold condition (10℃ ±1℃) in a cryogenic vessel,3 h/d,for 3 days.The mice of the YPF group were given YPF on day 7 before cold exposure until the end of the experiment.ILCs,and its three subsets (ILCI,ILC2,and ILC3) in lung lymphocytes were detected by flow cytometry.The expression of IFNγ,IL-13,and IL-17A was detected by RT-PCR.Results Compared with the control group,the proportions of ILCs,ILC1,ILC2,and ILC3 were lower in the hypothermia model group (all P ＜ 0.05) as well as the mRNA expression of IFFNγ,IL-13,and IL-17A (all P ＜0.01).Compared with the hypothermia model group,the proportions of ILCs,ILC1,ILC2,and ILC3 were higher in the YPF group (all P＜0.05),as well as the mRNA expression of IFNγ,IL-13,and IL-17A (all P ＜ 0.05).Conclusions Yupingfeng powder may reverse the inhibitory effect of hypothermia on ILCs and the functional factors in lung tissues.关键词
低温/固有淋巴样细胞/肺/玉屏风散/中药Key words
hypothermia/innate lymphoid cells/lung/Yupingfeng powder/traditional Chinese drugs分类
医药卫生引用本文复制引用
安磊,郭钰琪,王丽,孙晓艳,吕衍民,兰红云,姚成芳..玉屏风散对低温环境下小鼠肺脏固有淋巴样细胞比例及功能的影响[J].山东医药,2017,57(22):12-14,3.基金项目
国家自然科学基金资助项目(81573728) （81573728）
山东省自然科学基金资助项目(ZR2015HM007). （ZR2015HM007）
