山东医药2017，Vol.57Issue(22)：23-25,3.DOI:10.3969/j.issn.1002-266X.2017.22.007
TET1基因敲除的宫颈癌Hela细胞增殖、侵袭能力观察
Proliferation and invasion abilities of TET1 gene knock-out Hela cervical cancer cells
摘要
Abstract
Objective To observe the proliferation and invasion abilities of ten-eleven translocation 1 (TET1) gene knock-out Hela cervical cancer cells.Methods We constructed the TET1 knock-out Hela cells by CRISPR/Cas9 technology,which were defined as the observation group.At the same time,we defined wild Hela cells as the control group.We observed the proliferation ability of Hela cells in the observation and control group by the real-time cellular analysis (RTCA),and calculated the cell index (CI) values in each group at 90 h.We observed the invasion ability of Hela cells in each group by Transwell assay.Results The CI value of the observation group was 1.04 ± 0.86,which was higher than that of the control group (i.47 ± 1.01) (P ＜0.05).At 24 h,the transmembrane cells in the observation group and the control group were 158 ± 6 and 109 ± 4,respectively;at 48 h,the of transmembrane cells in each group were 206 ± 12 and 142 ± 7,respectively;significant difference was found between these two groups (P ＜ 0.05).Conclusion The proliferation and invasion abilities of TET1 gene knock-out Hela cells become stronger.关键词
宫颈癌/Hela细胞/TET1基因/CRISPR/Cas9技术/细胞增殖/细胞侵袭Key words
cervical carcinoma/Hela cells/TET1 gene/CRISPR/Cas9 technique/cell proliferation/cell invasion分类
医药卫生引用本文复制引用
沈凤,丁妍,谭玉洁..TET1基因敲除的宫颈癌Hela细胞增殖、侵袭能力观察[J].山东医药,2017,57(22):23-25,3.基金项目
国家自然科学基金资助项目(81602297) （81602297）
贵州省科技计划项目(黔科合基础2016-1121). （黔科合基础2016-1121）
