山东医药2017，Vol.57Issue(28)：5-8,4.DOI:10.3969/j.issn.1002-266X.2017.28.002
XPD基因在氢醌致U937细胞DNA损伤修复中的作用
Role of XPD gene in DNA damage repair of U937 cells induced by hydroquinone
摘要
Abstract
Objective To investigate the role of XPD gene in DNA damage repair of U937 cells induced by hydroquinone (HQ).Methods U937 cells were treated with 5, 10, 20, 40, and 80 μmol/L HQ for 12, 24 and 48 h.According to the cell activity, we selected 10, 20, and 40 μmol/L HQ for the following experiments.U937 cells were treated with 10, 20 and 40 μmol/L HQ for 24 and 48 h, which were taken as the low-dose, medium-dose, and high-dose HQ groups, meanwhile, the untreated U937 cells were taken as the blank control group.Single cell gel electrophoresis tail moment (TM) and Oliv tail moment (OTM) were used to detect DNA damage.The relative expression of XPD protein in each group was determined by Western blotting.Results Compared with the blank control group, TM and OTM in the low-dose, medium-dose, and high-dose HQ groups at 24, 48 h significantly increased (both P<0.05).TM and OTM in the low-dose, medium-dose, and high-dose HQ groups at 48 h significantly increased as compared with that of the same group at 24 h (both P<0.05), and the TM and OTM values of the low-dose, medium-dose, and high-dose HQ groups increased successively (both P<0.05).Compared with the blank control group, the relative expression of XPD protein in the low-dose, medium-dose, and high-dose HQ groups at 24 h was significantly higher (P<0.05), and the high-dose group was significantly higher than the low-dose group (P<0.05).The relative expression of XPD protein in the high-dose HQ group at 48 h was significantly higher than that in the blank control group, low-dose, and medium-dose HQ groups (P<0.05), but there was no significant difference between the blank control group and low-dose and medium-dose HQ groups (all P>0.05).The relative expression of XPD protein in each group at 48 h was not significantly different from that at 24 h (all P>0.05).Conclusion The up-regulation of XPD gene expression promotes the repair of DNA damage of U937 cells induced by HQ.关键词
DNA损伤/氢醌/XPD基因/DNA修复Key words
DNA damage/hydroquinone/XPD gene/DNA repair分类
生物科学引用本文复制引用
王永红,李攀登,肖芸,万秀方,郑丹,温缘缘,赵雪,张亚莉,杨国珍..XPD基因在氢醌致U937细胞DNA损伤修复中的作用[J].山东医药,2017,57(28):5-8,4.基金项目
国家自然科学基金资助项目(81360437) （81360437）
贵州省科教青年英才培养工程项目(黔省专合字〔2012〕164号). （黔省专合字〔2012〕164号）
