山东医药2017，Vol.57Issue(28)：28-32,5.DOI:10.3969/j.issn.1002-266X.2017.28.008
p38 MAPK在MRP8/14诱导小鼠胚胎成纤维细胞凋亡中的作用
Role of p38 MAPK on apoptosis of mouse embryo fibroblasts induced by MRP8/14
摘要
Abstract
Objective To explore the role of p38 MAPK on the apoptosis of mouse embryo fibroblasts induced by myeloid-related protein 8 (MRP8)/myeloid-related protein 14 (MRP14).Methods MTT assay was performed to detect the cell viability of p38+/+ and p38-/-cells in the control group and groups in which cells were treated with 50 μg/mL MRP8, MRP14, and MRP8/14 for 24 h and 48 h;flow cytometry was applied to detect the apoptosis rate of p38+/+ and p38-/-cells in the control group and groups in which cells were treated with 50 μg/mL MRP8/14 for 24 h and 48 h;MTT assay was performed to detect the cell viability of p38+/+ cells in the control group, MRP8/14 group, MRP8/14+TAK242(TLR4 inhibitor) group and MRP8/14+RAGE neutralized antibody group for 24 h;MTT and flow cytometry were used to analyze the cell viability and the apoptosis rate of p38+/+ cells in the control group, MRP8/14 group, MRP8/14+SB203580 (p38 MAPK inhibitor) group for 24 h;Western blotting was used to detect the phosphorylation changes of p38 MAPK in p38+/+ cells treated with MRP8/14 for 0, 1, 2, 4, 6, and 8 h, respectively.Results The cell viability of p38+/+ and p38-/-cells in the groups treated with MRP8, MRP14, and MRP8/14 for 24 h and 48 h was lower than that of the control group (P<0.05).At the same time, the cell viability of p38-/-cells was significantly higher than that of p38+/+ cells in the MRP8/14 group at 24 and 48 h (P<0.05).The apoptosis rate of p38+/+ cells in the MRP8/14 group at 24 and 48 h was higher than that of the control group, especially at 48 h (all P<0.05).However, the apoptosis rate of p38-/-cells did not change after MRP8/14 treatment for 24 and 48 h.The cell viability of p38+/+ cells in the MRP8/14 group and MRP8/14+RAGE neutralized antibody group was lower than that in the control group and MRP8/14 +TAK242 group (P<0.05).The cell viability of p38+/+ cells in the MRP8/14+SB203580 group was higher than that of the MRP8/14 alone group, and the apoptosis rate of p38+/+ cells in the MRP8/14+SB203580 group was lower than that of the MRP8/14 alone group (P<0.05).The phosphorylation level of p38 MAPK in p38+/+ cells was significantly higher at 2, 4, and 6 h after MRP8/14 treatment than that at 0, 1, and 8 h (P<0.05).Conclusion MRP8/14 can induce the apoptosis of mouse embryo fibroblasts via the activation of TLR4-p38 MAPK pathway.关键词
胚胎成纤维细胞/髓样相关蛋白8/髓样相关蛋白14/p38丝裂原活化蛋白激酶/细胞凋亡/小鼠Key words
embryo fibroblasts/myeloid-related protein 8/myeloid-related protein 14/p38 mitogen-activated protein kinase/apoptosis/mouse分类
医药卫生引用本文复制引用
王娟,陈小欢,李磊,卢夏英,姜勇..p38 MAPK在MRP8/14诱导小鼠胚胎成纤维细胞凋亡中的作用[J].山东医药,2017,57(28):28-32,5.基金项目
国家自然科学基金资助项目(81501691). （81501691）
