上海海洋大学学报2017，Vol.26Issue(4)：490-500,11.DOI:10.12024/jsou.20161101909
驯化硝化型生物絮体养殖南美白对虾的初步研究
Preliminary study on domesticating nitrifying bio-flocs to rear Litopenaeus vannamei
摘要
Abstract
This study tries to gradually reduce the addition of carbon source to zero,and to explore the effect of domesticating nitrifying bio-flocs on water quality,bacterial community dynamics of bio-floc and the growth performance of shrimp in high-intensive culture system.Experiment was carried out in three runway aquaculture systems at stocking density of 685 shrimp/m3.Water quality results showed that daily addition of glucose at 100%-150% of feed,which can decrease the concentration of TAN,but the concentration of NO2--N was not decreased obviously.When the addition of glucose dropped to zero,TAN can be maintained at lower level,and the NO2-N concentration decreased significantly.The bacterial community structure was analyzed by using high throughput sequencing technology.At the phylum level,experiment results showed that both the main advantage microflora of heterotrophic bioflocs and nitrifying bioflocs are Proteobacteria and Bacteroidetes.At the class level,dominant microflora of heterotrophic biofloc is Alphaproteobacteria,and dominant microflora of nitrifying bioflocs are Alphaproteobacteria,Flavobacteria and Gammaproteobacteria.After 65 d,study found that without adding carbon source,nitrifying bio-flocs can limit the abundance of heterotrophic bacteria,nitrospira was rising,and developing into dominant bacteria.At the end of the experiment, glucose which is 41.03%±7.86% of feed was added throughout the culture period.The survival rate and harvest of Litopenaeus vannamei respectively were 43.35%±7.57% and (3.03±0.59) kg/m3.The domestication of nitrifying bio-flocs not only saved cost, but also effectively optimized the structure of bacterial communities, and contributed to better water environment which results in the higher growth and survival of shrimp.关键词
生物絮凝养殖系统/硝化型生物絮体/南美白对虾/水质/生长/细菌群落Key words
bio-floc culture system/nitrifying bio-floc/Litopenaeus vannamei/water quality/growth/bacterial community分类
农业科技引用本文复制引用
谭洪新,庞云,王潮辉,罗国芝,刘文畅..驯化硝化型生物絮体养殖南美白对虾的初步研究[J].上海海洋大学学报,2017,26(4):490-500,11.基金项目
"十二五"农村领域国家科技计划课题(2012BAD25B03) （2012BAD25B03）
上海市科学技术委员会项目(16DZ2281200,14320501900) （16DZ2281200,14320501900）
