丝绸2017，Vol.54Issue(7)：1-6,6.DOI:10.3969/j.issn.1001-7003.2017.07.001
鲜茧生丝多酚微量组分提取方法
Extraction method of polyphenols trace components in fresh raw silk
摘要
Abstract
To explore the extraction method of trace components in fresh raw silk, this paper is intended to study the effect of extraction solvents (ethanol/water solution) of different mass fractions and extraction conditions (temperature, time and liquor ratio) on extraction efficiency of polyphenols trace components in fresh raw silk via high performance liquid chromatography (HPLC), figure out the optimum extraction conditions via orthogonal test.The results indicate that the HPLC chromatograms of extracts from fresh raw silk with different solvents significantly differ.Specifically, with the mass fraction of ethanol is in negative correlation with both the intensity of chromatographic peaks and the content of components.All the extraction conditions have impact on the content of components having retained for 10 to 16min, and the impact of temperature is most significant.Meanwhile, researches show that pure water is the best extraction solvent of polyphenols trace components in fresh raw silk, of which the optimum extraction conditions are: temperature of 40℃, duration of 90min, and liquor ratio of 1:25 (g/mL).关键词
生丝/鲜茧/多酚/提取/高效液相色谱Key words
raw silk/fresh cocoon/polyphenols/extract/HPLC分类
轻工纺织引用本文复制引用
王聪磊,马明波,潘璐璐,董锁拽,唐志荣,周文龙..鲜茧生丝多酚微量组分提取方法[J].丝绸,2017,54(7):1-6,6.基金项目
国家质检总局科技项目(2016IK282) （2016IK282）
