世界中医药2017，Vol.12Issue(6)：1391-1394,4.DOI:10.3969/j.issn.1673-7202.2017.06.045
探讨降糖消渴颗粒对高脂饮食诱导2型糖尿病肾脏氨基末端激酶信号通路的影响
Jiangtangxiaoke Granules on High Fat Diet Induced Type 2 Diabetic Kidney Amino Terminal Kinase Pathway
摘要
Abstract
Objective:To study the Jiangtangxiaoke granule in the treatment of high fat diet induced type 2 diabetes and to investigate its effect on renal N-terminal kinase signal pathway.Methods:All 72 Wistar rats were reared in SPF conditions,and were randomly divided into an intervention group,a control group and a normal control group,24 rats in each.By streptozotocin (STZ) intraperitoneal injection of 30 mg/kg synergy,high fat diet induced type 2 diabetes animal models were built.After the model intervention,the normal control group received standard diet;the control group was treated with high glucose and high fat diet;the intervention group treated with high glucose,high fat diet,and Jiangtangxiaoke granules were calculated according to the body weight of 9 g/kg rats by gavage once daily.Treatment lasted 8 weeks.Using enzyme-linked immunosorbent assay (ELISA method) to detect rats blood glucose (Glu),triglyceride (TG),total cholesterol (TC),serum creatinine (Scr);routine HE staining,observing the changes of renal structure in each group;real time fluorescent polymerase chain reaction (q-PCR) detection (amino terminal kinase) and JNK (glucagon like peptide-1 GLP-1) and Western blotting (Western Blot) to detect the activation of JNK signaling pathway,JNK (phosphorylated N-terminal kinase) p-JNK and GLP-1.Results:1) The Glu,TC,TG and Scr in the intervention group were significantly lower than those in the control group (P ＜ 0.05).2) Compared with the control group,the changes of glomerular structure were significantly improved in the intervention group (P ＜ 0.05).3) Compared with the normal control group,the expression of p-JNK protein in control group increased significantly (P ＜ 0.05),GLP-1 gene and protein significantly decreased (P ＜ 0.05);after treatment,the intervention group of p-JNK protein was lower than that of the control group (P ＜0.05),and GLP-1 (P ＜ 0.05) gene and protein increased.Conclusion:Jiangtangxiaoke granules can regulate sugar,lipid metabolism,protect the kidney function,its mechanism may be related to the inhibition of the JNK signaling pathway and regulation of GLP-1.关键词
降糖消渴颗粒/高脂饮食/2型糖尿病/肾脏/氨基末端激酶信号通路Key words
Jiangtangxiaoke granule/High fat diet/Type 2 diabetes mellitus/Kidney/N-terminal kinase signal pathway分类
医药卫生引用本文复制引用
常智跃,高思华,穆倩倩,左加成,赵丹丹,于娜..探讨降糖消渴颗粒对高脂饮食诱导2型糖尿病肾脏氨基末端激酶信号通路的影响[J].世界中医药,2017,12(6):1391-1394,4.基金项目
国家自然科学基金项目(编号:NSFC81503540) （编号:NSFC81503540）
