世界中医药2017，Vol.12Issue(6)：1407-1412,1416,7.DOI:10.3969/j.issn.1673-7202.2017.06.049
Th17/Treg细胞在健脾方防治克罗恩大鼠中的作用机制
The Mechanism of Th17/Treg Cells in The Prevention and Treatment for Rat with Crohn's Disease with a Spleen-invigorating Prescription
摘要
Abstract
Objective:To observe the mechanism of TH17/Treg cells in the prevention and treatment for trinitrobenzene sulfonic acid (TNBS)-induced rats with Crohn's disease using a spleen-invigorating prescription (SIP).Methods:A total of 24 SPF grade male Sprague-Dawley (SD) rats were randomly divided into 3 groups:(1) the normal rat control group (NG;n =8) received intragastrically administered double-distilled water once daily at 1 mL/100 g for 3 weeks;(2) the TNBS model group (MG;n =8) received enemas with TNBS once weekly at a dosage of body weight (g) × 0.003 ml/g for 4 weeks;and (3) the TNBS model + SIP and treatment group (JP;n =8) was treated with an additional Chinese SIP 1 week after undergoing TNBS enema.The Chinese medicine was administrated intragastrically once daily for another 3 weeks at a dosage of 1 mL/100 g.Multiple techniques,including enzyme-linked immunosorbent assay(ELISA),immunohistochemistry,and real-time PCR,were used to observe the differential expression of particular genes and proteins in blood and colonic mucosa of rats which belong to different groups.This study focused on genes and proteins that are highly associated with Thl7 and Treg cell differentiation and function,including interferon (INF)-γ,IL-17,retinoid-related orphan nuclear receptor (ROR) γt,and FoxP3.Results:SIP effectively adjusted Th1/Th2 homeostasis by downregulating the Th1-secreted cytokines INF-γ.By downregulating Thl7-secreted inflammatory factors,IL-17 as well as RORγt and suppressing the secretion of FoxP3,SIP also promoted Th17/Treg homeostasis.Then,the symptoms of TNBS-induced rat colonitis were successfully prevented and treated.Conclusion:SIP prevented and treated inflammation associated with TNBS-induced rat with Crohn's disease by regulating multiple key cytokines and transcription factors related to Th1/Th2 and Th17/Treg cell homeostasis.关键词
克罗恩病/健脾方/IFN-γ/IL-17/RORγt/FoxP3Key words
Colonitis/Spleen-invigorating/IFN-γ/IL-17/RORγt/FoxP3分类
医药卫生引用本文复制引用
吴璐一,翁志军,季光..Th17/Treg细胞在健脾方防治克罗恩大鼠中的作用机制[J].世界中医药,2017,12(6):1407-1412,1416,7.基金项目
上海市优秀青年教师项目(编号:ZZszy12011) （编号:ZZszy12011）
