生命科学研究2017，Vol.21Issue(3)：195-200,6.DOI:10.16605/j.cnki.1007-7847.2017.03.002
壳寡糖对神经细胞的抗氧化保护作用研究
Antioxidative Effect of Chitosan Oligosaccharide on Nerve Cells
摘要
Abstract
Oxidative damage of nerve cells is one of the most important causes leading to neurodegenerative diseases including Alzheimer's disease (AD).To investigate the protection mechanism of chitosan oligosaccharide (COS) on nerve cells,hydrogen peroxide was employed as a source of oxidative stress to set up an injury model for SH-SY5Y cells.MTT assay,cellular MDA level and LDH releases,Hoechst 33342 fluorescence staining and flow cytometry were conducted to examine the cellular apoptosis.Then,Western-blot was carried out to analyze the expression of apoptosis-related proteins and further investigate the effect of COS against hydrogen peroxide-induced oxidation.The results showed that COS could effectively improve the cellular survival rate,inhibit LDH releases and repress MDA increase under hydrogen peroxide stress.Besides,COS exhibited repressive effect against hydrogen peroxide-mediated apoptosis,and decreased the ratio of Bax/Bcl-2.These results suggest that the neuroprotective mechanism of COS on relieving oxidative stress induced by hydrogen peroxide may be related to its antioxidative effect,alleviation of lipid peroxidation damage and inhibition of cell apoptosis.关键词
壳寡糖(COS)/过氧化氢(H2O2)/SH-SY5Y神经细胞/神经退行性疾病/氧化损伤/细胞凋亡Key words
chitosan oligosaccharide (COS)/hydrogen peroxide (H2O2)/SH-SY5Y neuroblastoma cell/neurodegenerative disease/oxidative damage/cell apoptosis分类
生物科学引用本文复制引用
戴雪伶,常平,黄汉昌,窦慧娟,姜招峰..壳寡糖对神经细胞的抗氧化保护作用研究[J].生命科学研究,2017,21(3):195-200,6.基金项目
北京市自然科学基金资助项目(6164030) （6164030）
国家自然科学基金资助项目(31071512) （31071512）
北京市教委科技计划面上项目(SQKM201511417013) （SQKM201511417013）
北京市优秀人才资助项目(2015000020124G049) （2015000020124G049）
