生命科学研究2017，Vol.21Issue(3)：201-207,7.DOI:10.16605/j.cnki.1007-7847.2017.03.003
绿色杜氏藻异戊烯基焦磷酸异构酶基因(DvIPI)的生物信息学与诱导表达分析
Bioinformatic Analysis and Induced Expression of Isopentenyl Diphosphate Isomerase Gene (DvIPI) from Dunaliella viridis
摘要
Abstract
Isopentenyl diphosphate isomerase (DvIPI) is an important rate-limiting enzyme involved in mevalonate synthesis pathway of Dunaliella viridis,which limits the biosynthesis of terpenoids such as carotenoid.Here,the full-length cDNA sequence of IPI gene (GenBank number.KX189195) in D.viridis was obtained and its protein structure and function were analyzed.The full-length IPI gene of D.viridis was 4 641 bp long,had an open reading frame of 1 147 bp with 3 CBS domains and encoded 382 amino acids.The protein prediction results showed that DvIPI was hydrophilic,without a signal peptide and transmembrane domain,and had the highest proportion of alpha helix and irregular twist in the middle.What's more,both sides of DvIPI had a twisted polypeptide chain far from the center.According to phylogenetic analysis,DvIPI had a close homologous relationship with bacterial IPI,but had little relationship with those of angiosperms and other algae.Quantitative detection and RT-PCR showed that the effects on carotenoid and chlorophyll contents and the expression level of IPI in D.viridis were the most obvious when MeJA concentration was between 50 μmol/L and 100 μmol/L,and that their trends were basically the same.This suggests that the induction of carotenoid and chlorophyll by MeJA might be associated with IPI gene in D.viridis.关键词
绿色杜氏藻/异戊烯基焦磷酸异构酶基因(IPI)/生物信息学/CBS结构域蛋白(CDCP)/茉莉酸甲酯(MeJA)Key words
Dunaliella viridis/isopentenyl diphosphate isomerase (IPI) gene/bioinformatics/CBS domain containing protein (CDCP)/methyl jasmonate (MeJA)分类
生物科学引用本文复制引用
陈俊粤,龚一富,马颖瑞,周丽亚,王何瑜..绿色杜氏藻异戊烯基焦磷酸异构酶基因(DvIPI)的生物信息学与诱导表达分析[J].生命科学研究,2017,21(3):201-207,7.基金项目
浙江省科技厅重点科技创新团队项目(2012R10029-07,2010R50029) （2012R10029-07,2010R50029）
宁波科技攻关项目(2013C10018) （2013C10018）
