生命科学研究2017，Vol.21Issue(3)：213-219,7.DOI:10.16605/j.cnki.1007-7847.2017.03.005
人细胞角蛋白Keratin14的生物信息学分析
Bioinformatic Analysis of Human Keratin 14 Gene and Protein
摘要
Abstract
Keratin 14 is a member of the type I intermediate filament protein family,and involves in the formation of epithelial cell cytoskeleton.Here,bioinformatic analysis was performed to analyze the promoter and CpG islands of Keratin 14 gene,as well as the physical and chemical properties,structural and functional characteristics of Keratin 14 protein.The results showed that there are two promoters and no CpG island in the gene.In addition,Keratin 14 protein is composed of 472 amino acids,with isoelectric point of 5.09,and is a hydrophilic protein without a signal peptide and transmembrane domain.Furthermore,seven α-helices and fiveβ-sheets were predicted in the secondary structure,and the structure was proven to be stable by Ramachandran plot.From the function perspective,Keratin 14 not only interacts with Keratin but also plays an essential role in regulating cell growth and division,prompting cell differentiation.The homologies of the amino acid sequence to those of other mammals are very high.The analysis results provide important messages for further studies on function of Keratin 14 in human life activities.关键词
角蛋白Keratin 14/上皮细胞/细胞骨架/肿瘤/生物信息学Key words
Keratin 14/epithelial cell/cytoskeleton/cancer/bioinformatics分类
生物科学引用本文复制引用
周蒙,董秋萍,张连民,李伟,付欣,许世磊..人细胞角蛋白Keratin14的生物信息学分析[J].生命科学研究,2017,21(3):213-219,7.基金项目
国家自然科学基金资助项目(81301711) （81301711）
