生命科学研究2017，Vol.21Issue(3)：268-274,7.DOI:10.16605/j.cnki.1007-7847.2017.03.014
基因编辑技术的研究进展
Research Advances in Gene Editing Technology
李妤 1赵红业 2崔勇 3魏红江 2李梅章1
作者信息
- 1. 云南大学生命科学学院,中国云南昆明650091
- 2. 云南农业大学动物科学学院,中国云南昆明650201
- 3. 云南农业大学农学与生物技术学院,中国云南昆明650201
- 折叠
摘要
Abstract
The most widely used approach for defining gene function is to reduce or completely disrupt its normal expression.Many gene editing tools are used widely in recent years,including RNAi technology and some increasingly developed technologies with efficient enzymes,such as zinc finger nuclease (ZFN),transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (CRISPR/Cas9).In addition to above technologies,NgAgo is also a new gene editing tool published in 2016.For over a decade,gene editing tools represented by the CRISPR/Cas9 technique have been widely used in gene knockout research based on the cellular level and individual level.Here,the related principles and the latest research progresses of these technologies are summarized,and the principle of CRISPR/Cas9 technology and its latest progress in gene knockout animal models are highlighted.关键词
基因敲除/RNA干扰(RNAi)/锌指核酸酶(ZFN)/转录激活样效应物核酸酶(TALENs)/成簇的规律间隔的短回文重复序列系统(CRISPR/Cas9)/Argonaute内切酶分类
生物科学引用本文复制引用
李妤,赵红业,崔勇,魏红江,李梅章..基因编辑技术的研究进展[J].生命科学研究,2017,21(3):268-274,7.
