食品与发酵工业2017，Vol.43Issue(6)：34-40,7.DOI:10.13995/j.cnki.11-1802/ts.201706006
地衣芽胞杆菌碱性蛋白酶嗜碱突变体的特征分析
Biochemical characterization of alkalophilic mutant of Bacillus licheniformis protease
摘要
Abstract
Alkaline protease from Bacillus licheniformis is an important protease in the industry.Improving its activity and stability under alkaline pH conditions can broaden the range of applications in detergent industry.In this study,a high-alkaline protease from B.licheniformis B186 was screened,and its encoding gene was successfully cloned and expressed in B.subtilis WB600 to generate the recombinant bacterium WB600 (pHY-E209).Recombinant alkaline protease AprE209 was then purified to apparent homogeneity from the supernatant of the recombinant bacterium using ion exchange chromatography and filtration chromatography.The optimum pH and temperature of recombinant enzyme AprE209 were 11.0 and 50 ℃,respectively.This recombinant enzyme also retained high activities at temperatures of 30-37 ℃ or pH 12.0.Furthermore,the mechanism of alkali-tolerance of AprE209 was analyzed by bioinformatics tools.The alkalophilic mutant of B.licheniformis protease obtained in this study had potential applications in detergent industry.关键词
蛋白酶/嗜碱性突变体/洗涤剂行业/酶学特征Key words
protease/alkalophilic mutant/detergent industry/enzymatic properties引用本文复制引用
黄磊,董自星,金鹏,王正祥,路福平..地衣芽胞杆菌碱性蛋白酶嗜碱突变体的特征分析[J].食品与发酵工业,2017,43(6):34-40,7.基金项目
国家自然科学基金面上项目(31370076) （31370076）
福建省自然科学基金(2016J01157) （2016J01157）
