中国生态农业学报2017，Vol.25Issue(8)：1198-1205,8.DOI:10.13930/j.cnki.cjea.170065
Bt基因导入对侵染丛枝菌根真菌的玉米生长生理及磷转运基因表达的影响
Effect of Bt gene insertion on growth, physiology and gene expression of phosphorus transporter gene of corn after arbuscular mycorrhizal fungi colonization
摘要
Abstract
Bt (Bacillus thuringiensis)-corn root can exudate insecticide active Bt protein and persistent toxin in soils which can affect symbiotic relationship between corn and arbuscular mycorrhizal fungi (AMF). In this paper, two Bt-corn varieties '5422Bt1' (Bt11) and '5422CBCL' (Mon810), and one conventional corn variety '5422' were used to explore the difference between Bt and non-Bt varieties with Funneliformis mosseae inoculation and non-inoculation treatments. The colonization of AMF, phosphorus transporter gene expression, growth and nutrients contents were measured after 50 d and 80 d of inoculation. The results showed that AMF colo-nization of Bt-corn varieties '5422Bt1' and '5422CBCL' were significantly higher than that of the conventional variety '5422' at 50 d and 80 d after treatment, which increased by 13.54% and 11.24% at 50 d and then by 9.83% and 6.70% at 80 d, respectively. There was no significant difference in phosphorus transporter gene expression between inoculation and non-inoculation for Bt-corn varieties at 50 d. The height of '5422' under inoculation treatment was significantly higher than that of '5422Bt1' and '5422CBCL' at 50 d. Dry weight of '5422Bt1' under inoculation treatment was significantly higher than that of '5422CBCL' at 50 d. The root length, root surface and root volume of '5422Bt1' were significantly higher than those of '5422' and '5422CBCL' under non-inoculation treatments at 80 d. Bt gene insertion affected nitrogen concentration of the two Bt-corn varieties ('5422Bt1'and '5422CBCL') at the two sampling times. As compared with conventional corn variety, the two Bt-corn varieties needed more nitrogen and phosphorus to synthetize Bt protein. There were significant differences among the responses of three corn varieties to AMF inoculation. F. mosseae inoculation signifi-cantly increased nitrogen absorption of'5422Bt1' and '5422CBCL' at the two sampling times (50 d and 80 d). In conclusion, the ef-fect of Bt-gene insertion and AMF inoculation on growth and nutrient utilization of Bt-corn was mainly dependent on its transforma-tion event.关键词
Bt玉米/丛枝菌根真菌/生长特性/养分利用/磷转运基因Key words
Bt-corn/Arbuscular mycorrhizal fungi/Growth characteristics/Nutrient utilization/Phosphorus transporter gene分类
农业科技引用本文复制引用
钟旺,曾慧兰,王建武..Bt基因导入对侵染丛枝菌根真菌的玉米生长生理及磷转运基因表达的影响[J].中国生态农业学报,2017,25(8):1198-1205,8.基金项目
国家自然科学基金项目(31470574, 31170506)资助 This study was founded by the National Natural Science Foundation of China (31470574, 31170506). （31470574, 31170506）
