山西农业大学学报（自然科学版）2017，Vol.37Issue(8)：570-574,5.
河岸林土壤好氧反硝化菌的分离及活性初探
Study on isolation and denitrification activity of aerobic denitrifiers from a riparian forest
摘要
Abstract
[Objective]Aerobic denitrifiers could decrease nitrate and release N2O, and reduce the harm of rich nitrogen soil under aerobic conditions. The study was conducted to verify the existence of aerobic denitrifiers in riparian forest soil.[Methods]In this study, the soil of riparian forest adjacent to farmland from the upper reaches of Wenyu River was taken as material, using soil dilution, plate streak, adding griess and diphenylamine colorimetric reactionmethod for strain screening, 16S rDNA analysis for identification and determination. The denitrificative activity was analyzed by determination the nitrate, ammonium, and nitrite content in sterilized soil inoculated with the strains.[Result]3 strains of denitrifiers were isolated and screened, which was coded df3, df7and df14, were respectively identified and named as Psueadomonas sp.DF3,Alicaligenes sp.DF7, and Klebsiella sp.DF14. Denitrification activity analysis showed that the soil nitrate was obviously decreased, and the soil nitrite was produced inoculation of df3 and df7.[Conclusion]There were aerobic denitrifiers in the soil samples. It has important scientific significance of the study.关键词
河岸林/好氧反硝化细菌/筛选/反硝化活性Key words
Riparian forest/Aerobic denitrifying bacteria/Screening/Denitrification activity分类
生物科学引用本文复制引用
韩晓丽,聂园军,黄春国,郭晋平..河岸林土壤好氧反硝化菌的分离及活性初探[J].山西农业大学学报（自然科学版）,2017,37(8):570-574,5.基金项目
山西农业大学科技创新基金(20132-14) （20132-14）
