山西农业大学学报（自然科学版）2017，Vol.37Issue(9)：635-639,5.
毛梾腋芽诱导关键因子研究与培养体系优化
The study of the key factors affecting axillary bud induction and optimizing system of cornus walteri
摘要
Abstract
[Objective]To establish an efficient system for axillary bud induction using young stem segments as explants of Cornus walteri.[Methods]The paper studied the influence factors which included the disinfectant types and the disinfection time, the collection period of explants, the types of basic culture medium, the combination of plant growth regulators, pH et al.[Result](1) The best disinfectant and disinfecting time was 0.1% mercuric chloride for 4~5 min;(2) The better collection period for explants was from April to May;The best collection period was from January to March, using young stem segments as explants after water culture;(3) The optimum medium was MS medium for axillary bud induction;(4) The frequency of axillary bud induction were the highest when the medium supplemented with 0.5~1.0 mg·L-1 BA and 0.1 mg·L-1 NAA and 0.1 mg·L-1 IBA with 85.5% and 84.4%;(5) The optimum pH was 5.8 for axillary bud induction;(6) Light culture was more beneficial for the induction and development of axillary bud.[Conclusion]Using young stem segments as explants after water culture, 0.1% mercuric chloride as disinfectant for 4~5 min, cultured on MS supplemented with 0.5~1.0 mg·L-1 BA and 0.1 mg·L-1 NAA and 0.1 mg·L-1 IBA, were the best culture system for axillary bud induction of Cornus walteri.关键词
毛梾/茎段外植体/腋芽诱导Key words
Cornus walteri/Stem segment explant/Axillary bud induction分类
农业科技引用本文复制引用
张娜..毛梾腋芽诱导关键因子研究与培养体系优化[J].山西农业大学学报（自然科学版）,2017,37(9):635-639,5.基金项目
山西省科技攻关项目(20140311015-1) （20140311015-1）
山西省林业科技创新项目(2016) （2016）
