山西农业大学学报（自然科学版）2017，Vol.37Issue(9)：649-655,7.
百合S-RNase基因cDNA全长克隆及表达分析
Clone and expression analysis of the full-length cdna sequences of S-RNase in Lilium
摘要
Abstract
[Objective]The clone and the expression analysis of the full length sequence of Lilium S-RNase gene which would made a foundation for discovering the molecular regulation of incompatibility in Lilium.[Methods]The full cDNA sequence of S-RNase was isolated from a style of Lilium oriental ''Justina'' by RACE.Semi-quantitative RT-PCR technology was used to analyse the expression of different organs of the S-RNase gene in the same period.Bioinformatic analysis was used by the online tools of National Center for Biotechnology Information and ProtParam, SignalP, MEGA5.[Results]The result showed that the full length of the S-RNase gene was 766 bp, with an open reading frame of 672bp, which encoded a protein with 223 amino acids.The semi-quantitative expression showed that its expression order from higher to lower were petals, leaves, stems, BL2, BL1, bulbs.It was predicted that this S-RNase protein was a hydrophilic proteins with a signal peptide,which belonging to the secrete protein.This protein had a typical conserved domains of RNase T2 family.The secondary structure was rich in loop structure.The relationship analysis showed that Camellia sinensis was closest with Lilium among the known S-RNase gene.[Conclusion]The full length sequence of Lilium S-RNase gene was obtained and the expression of different organs of the S-RNase gene in same period were analysed by semi-quantitative RT-PCR technology.关键词
百合/S-RNase基因/克隆/半定量Key words
Lilium/S-RNase gene/Cloning/Semi quantitative RT PCR分类
农业科技引用本文复制引用
丁榕,王杰,赵和文,张克中,崔金腾..百合S-RNase基因cDNA全长克隆及表达分析[J].山西农业大学学报（自然科学版）,2017,37(9):649-655,7.基金项目
北京市教育委员会2015年科技计划面上项目(KM201510020011) （KM201510020011）
北京市属高等学校创新团队建设项目(IDHT20150503) （IDHT20150503）
科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心(2011协同创新中心)(市级)(PXM2017_014207_000043) （2011协同创新中心）
北京农学院学位与研究生教育改革与发展项目(5056516004/026) （5056516004/026）
