实用妇产科杂志2017，Vol.33Issue(6)：420-425,6.
蜕膜基质细胞培养液对滋养细胞增殖、凋亡及浸润的影响及对浸润作用机制的研究
Impact of Conditioned Medium of Decidual Stromal Cell Cluture on Proliferation,Apoptosis and Invasion of Trophoblast Cells
摘要
Abstract
Objective:To investigate the impact of conditioned medium of decidual stromal cell culture(DSCM) on proliferation,apoptosis and invasion of trophoblast cells.Methods:lsolution,culturing and indentifying the purity of decidual stromal cells from healthy women of early pregnancy to prepare different concentrations of DSCM (5％,20％,50％ and 100％);Proliferation was evaluated by MTT assay;Cell apoptosis was examined by flow cytometry using annexin V-FITC/PI staining;Cell invasion was assayed using a Transwell chamber;RT-PCR and gelatin zymography were used to explore the mechanism of cell invasion.Results:Using this method we obtained a purity over 90％ of decidual stromal cells.With the concentration of DSCM increased(5％,20％,50％,100％),the OD value of the MTT assay has elevated and the rate of apoptosis declined,the infiltrating cell increased as well (P ＜ 0.05);Compared with control group,DSCM treatment led to a significant increase in the mRNA and protein expression of MMP-2/9(P ＜0.05).Conclusions:Decidual microenviroment may promote trophoblast proliferation and inhibite its apoptosis besides could enhance its ability of invasion by altering the expression of MMP-2 and MMP-9.关键词
蜕膜基质细胞/滋养细胞/增殖/凋亡/浸润Key words
Decidual stromal cells/Trophoblastic cells/Proliferation/Apoptosis/Invasion分类
医药卫生引用本文复制引用
康玲,尹国武,朱晓明..蜕膜基质细胞培养液对滋养细胞增殖、凋亡及浸润的影响及对浸润作用机制的研究[J].实用妇产科杂志,2017,33(6):420-425,6.基金项目
国家自然科学基金(编号:81471474) （编号:81471474）
唐都医院创新基金(编号:2015LCYJ013) （编号:2015LCYJ013）
