西安交通大学学报（医学版）2017，Vol.38Issue(4)：479-486,8.DOI:10.7652/jdyxb201704003
雌激素对大鼠肾缺血再灌注损伤后肾叶间动脉舒缩功能及Cx43表达的影响
Effects of estrogen on the vasomotor function of rats' renal interlobar arteries and Cx43 expression after renal ischemia-reperfusion injury
摘要
Abstract
Objective To investigate the effect of estrogen (E2) on the connexin43 (Cx43) expression of renal interlobar arteries after renal ischemia-reperfusion injury (I/R).Methods The experiment was carried out in vivo using an SD rat I/R model.SD rats were randomly divided into normal group,sham-operation group,I/R group,and estrogen-intervention group.The functional changes of the kidney were analyzed after 24 hours of I/R;nephridial tissue section was stained by hematoxylin-eosin (HE),and Paller scores were used to evaluate the degree of kidney damage.Pressure myography was utilized to detect the vasomotor function of renal interlobar arteries.Immunofluorescence technique,qRT-PCR and Western blot were applied to determine the expression of Cx43 in renal interlobar arteries in different groups.Results Estrogen markedly decreased the levels of Cr and BUN in the serum of I/R rats (P＜0.05),and the damage of the kidney tissue could be improved noticeably.The vasomotor rate of renal interlobar arteries was (24.80 ± 3.70)％ after I/R and (41.60 ± 3.50)％ after treatment with estrogen,which was higher than that of I/R group (P＜0.05).The expression of Cx43 was lower in renal interlobar arteries of estrogen-intervention group than that in I/R group (P＜0.01).Conclusion Estrogen may reduce vascular tension and boost dilation of the artery by inhibiting Cx43 expression and GJ function.Therefore,estrogen may attenuate the damage of I/R and improve renal function.关键词
雌激素/肾缺血再灌注损伤/肾叶间动脉/缝隙连接/Cx43Key words
estrogen/renal ischemia-reperfusion injury/renal interlobar artery (RIA)/gap junction/Cx43分类
医药卫生引用本文复制引用
冀伟,王卫民,常越辰,王洋,张亮,马克涛,司军强,李丽,李应龙..雌激素对大鼠肾缺血再灌注损伤后肾叶间动脉舒缩功能及Cx43表达的影响[J].西安交通大学学报（医学版）,2017,38(4):479-486,8.基金项目
国家自然科学基金资助项目(No.81560175,81560081,31260247)Supported by the National Natural Science Foundation of China (No.81560175,81560081 and 31260247) （No.81560175,81560081,31260247）
