西安交通大学学报（医学版）2017，Vol.38Issue(4)：492-496,5.DOI:10.7652/jdyxb201704005
下调尾型同源盒基因-2对结肠癌细胞的侵袭及迁移能力的影响及其机制
The effect and underlying mechanisms of down-regulated CDX2 on migration and invasion of colon cancer cells
摘要
Abstract
Objective To observe the effect of down-regulated CDX2 gene on the migration and invasion abilities of colon cancer cells (SW480 and HT29) and investigate the role and mechanisms of CDX2 gene in occurrence and development of colon cancer metastasis.Methods CDX2 gene in HT29 and SW480 cells was down-regulated using lentivirus RNA interference (RNAi) vector.The interference efficiency of CDX2 was detected by qRT-PCR and Western blotting.The effect of down-regulated CDX2 expression on colon cancer cells'migration and invasion was determined by Transwell and wound heal methods.Then the effects of down-regulated CDX2 on the expressions of epithelial-mesenchymal transition (EMT)-related genes (E-cadherin,ZEB-1,Vimentin,Twist and Snail) were detected by RT-PCR and Western blotting.Results The constructed CDX2 siRNA expression vector could significantly inhibit the expression of CDX2 in HT29 and SW480 cells.Compared with those of the cells transfected with empty vector (LV-NT-shRNA) and non-transfected cells,the migration and invasion abilities of cells transfected with LV-CDX2-shRNA were markedly enhanced (P ＜ 0.05).E-cadherin expression was reduced while expressions of ZEB-1,Vimentin,Twist,and Snail were significantly increased (all P＜0.05).Conclusion Down-regulating the expression of CDX2 can induce the occurrence of EMT,thus enhancing the invasion and migration of colon cancer cells.关键词
尾型同源盒基因-2(CDX2)/结肠癌/侵袭/迁移/上皮-间质转化(EMT)Key words
CDX2/colon cancer cell/invasion/migration/epithelial-mesenchymal transition (EMT)分类
医药卫生引用本文复制引用
郑见宝,祁杰,王训凯,李孝彬,王孝珑,陈南征,余钧辉,韩阳,孙学军..下调尾型同源盒基因-2对结肠癌细胞的侵袭及迁移能力的影响及其机制[J].西安交通大学学报（医学版）,2017,38(4):492-496,5.基金项目
国家自然科学基金资助项目(No.81101874 （）
No.81172362) （）
陕西省科学技术研究发展计划项目(No.2016SF-015 （）
No.2015SF037) （）
陕西省科技统筹创新工程计划项目(No.2013 KTCQ03-08)Supported by the National Natural Science Foundation of China (No.81101874 and No.81172362),Science and Technology Project of Shaanxi Province (No.2016SF-015 and No.2015SF037),and Shaanxi Provincial Coordinative and Innovative Plan Projects of Science and Technology (2013KTCQ03-08) （No.2013 KTCQ03-08）
