西安交通大学学报（医学版）2017，Vol.38Issue(4)：512-516,5.DOI:10.7652/jdyxb201704009
六磷酸肌醇对人骨肉瘤细胞株MG-63和原代培养成骨细胞增殖的影响
Effects of inositol hexaphosphate on proliferation of human osteosarcoma cell line MG-63 and primary cultured osteoblasts
摘要
Abstract
Objective To investigate the effects of inositol hexaphosphate (IP6) on proliferation of human osteosarcoma cell line MG-63 and primary cultured osteoblasts so as to explore the optimal concentration for achieving anti-cancer effects.Methods We primary cultured and identified human osteoblasts.Then we made recovery and normal culture of human osteosarcoma cell line MG-63.We tested the proliferation of two kinds of cell lines under different concentrations of IP6 by MTT to determine the optimal concentration and then detected MG-63 cell cycle and apoptosis by flow cytometry.Results When IP6 concentration was more than 1 mmol/L,IP6 began to inhibit the proliferation of MG-63 cell line in the time-dose dependent manner.When the concentration reached 4 mmol/L,this inhibitory effect was the maximum.When IP6 concentration was 0.5 mmol/L or 1 mmol/L,the proliferation of osteoblasts was not obviously inhibited.When it was 2 mmol/L,the proliferation was slightly inhibited.A concentration of 4 mmol/L caused the apoptosis of osteoblasts.Conclusion IP6 can inhibit the proliferation of osteosarcoma cell line MG-63 and lead to its apoptosis.The optimal concentration is 2 mmol/L for achieving anti-cancer effects.关键词
六磷酸肌醇(IP6)/骨肉瘤细胞株/成骨细胞/增殖/凋亡Key words
inositol hexaphosphate/osteosarcoma cell line/osteoblast/proliferation/apoptosis分类
医药卫生引用本文复制引用
戴星,郭池华,韩学哲,马巍..六磷酸肌醇对人骨肉瘤细胞株MG-63和原代培养成骨细胞增殖的影响[J].西安交通大学学报（医学版）,2017,38(4):512-516,5.基金项目
中央高校基本科研业务费专项资金资助项目(No.XJJ2015024) （No.XJJ2015024）
西安交通大学第一附属医院院基金资助项目(No.2013YK19) Supported by the Fundamental Research Funds for the Central Universities (No.XJJ2015024) and the Funds of the First Affiliated Hospital of Xi'an Jiaotong University (No.2013YK19) （No.2013YK19）
