西安交通大学学报（医学版）2017，Vol.38Issue(4)：536-539,548,5.DOI:10.7652/jdyxb201704013
肿瘤转移抑制基因-1在食管鳞癌组织及食管癌EC109细胞中的表达及意义
Expression and significance of TMSG-1 in esophageal squamous carcinoma and EC109 cells
摘要
Abstract
Objective To study the expression and significance of tumor metastasis suppressor gene-1(TMSG1) in esophageal squamous cell carcinoma (ESCC) and EC109 cells.Methods Immunohistochemistry S-P method was used to examine the expression of TMSG-1 protein in 136 cases of ESCC and 37 cases of normal esophageal mucosa.We analyzed the relationship between TMSG-1 and clinicopathological data of ESCC patients.EC109 cells were treated with 3 μg/mL of cisplatin (CDDP) in vitro for 24 h (the intervention group) and the control group was set up at the same time.The proliferation-inhibitory capability was analyzed with MTT assay.RT-PCR was used to examine the expression of TMSG-1 in the intervention group and the control group.Results The positive rate of TMSG-1 in ESCC and normal esophageal mucosa was 52.2％ (71/136) and 94.6％ (35/37),respectively.The expression of TMSG-1 in ESCC was significantly lower than that in normal esophageal mucosa (P＜0.05).The expression of TMSG-1 was related to TNM stage,differentiation degree and lymph node metastasis (P＜0.05).After EC 109 cells were treated with CDDP for 24 h,the proliferation inhibition rate was increased significantly compared with the control group (P＜0.01).RT-PCR results showed that the expression of TMSG-1 in the cells of the intervention group was significantly higher than that in the control group (P＜ 0.01).Conclusion The abnormal expression of TMSG-1 may play a role in the development and metastasis of ESCC.Examination of TMSG-1 may be useful for making diagnosis and guiding clinical therapy of ESCC.关键词
食管鳞状细胞癌/肿瘤转移抑制基因-1 (TMSG-1)/EC109细胞/免疫组织化学/RT-PCR/细胞增殖/顺铂(CDDP)Key words
esophageal squamous cell carcinoma/tumor metastasis suppressor gene-1 (TMSG-1)/EC109 cell/immunohistochemistry/RT-PCR/cell proliferation/cisplatin (CDDP)分类
医药卫生引用本文复制引用
刘博,吕洋,林媛媛,刘军超,李秀娟,乔海霞..肿瘤转移抑制基因-1在食管鳞癌组织及食管癌EC109细胞中的表达及意义[J].西安交通大学学报（医学版）,2017,38(4):536-539,548,5.基金项目
河北省科技厅计划项目(No.15277707D),河北省卫生厅科学技术指导项目(No.ZD20140220) Supported by the Planned Projects of the Science and Technology Department of Hebei Province (No.15277707D) （No.15277707D）
the Science and Technology Project of Hebei Provincial Department of Health (No.ZD20140220) （No.ZD20140220）
