厦门大学学报（自然科学版）2017，Vol.56Issue(4)：505-510,6.DOI:10.6043/j.issn.0438-0479.201607004
酶联免疫吸附分析双抗夹心法检测临床鼻咽抽吸物中呼吸道合胞病毒
Double-antibody Sandwich ELISA for Detecting Respiratory Syncytial Virus in Clinical Nasopharynx Aspirate Samples
摘要
Abstract
Respiratory syncytial virus (RSV) is the major cause of hospitalization and medically attended lower respiratory tract infection in children under 5 years old and infects all children by the age of 2.Currently,RSV infected patients are usually detected with the seven respiratory virus diagnostic kit (immunofluorescence,Diagnostic Hybrids company,USA),which is expensive and judged subjectively.Nowadays,a rapid diagnostic kit for detection of RSV is not available in China.In this study,we established a sandwich ELISA rapid diagnostic method for antigen detection of RSV antigen at the early stage of infection.112 clinical nasopharyngeal aspirate (NPA) samples were detected by sandwich ELISA and fluorescence microscopy diagnosis,with standard RT-PCR method as reference,and the results show that both methods have high consistency with the RT-PCR method.The sensitivity of the sandwich ELISA and fluorescence microscopy diagnosis was respectively 79.31% and 74.14%,with the specificity of 100% and 96.30%,as well as the Kappa value of 0.787 1 and 0.689 5.There was no statistically significant difference between sandwich ELISA and fluorescence microscopy diagnosis (p>0.05).In conclusion,our sandwich ELISA method is simple and highly specific,which is expected to replace imported testing kits for rapid diagnosis of early RSV infection.关键词
呼吸道合胞病毒/融合蛋白/酶联免疫吸附分析双抗夹心法/快速诊断Key words
respiratory syncytial virus (RSV)/fusion protein/double-antibody sandwich ELISA/rapid diagnosis分类
医药卫生引用本文复制引用
孙永鹏,靳输梅,詹炉停,温桂平,赵敏,张伟,夏宁邵,郑子峥..酶联免疫吸附分析双抗夹心法检测临床鼻咽抽吸物中呼吸道合胞病毒[J].厦门大学学报（自然科学版）,2017,56(4):505-510,6.基金项目
国家自然科学基金(81401668) （81401668）
NSFC-NIH生物医学合作研究项目(812111615) （812111615）
福建省科技创新平台建设计划项目(2014Y2101) （2014Y2101）
