厦门大学学报（自然科学版）2017，Vol.56Issue(4)：517-524,8.DOI:10.6043/j.issn.0438-0479.201610003
骆驼肠道内3种含氮杂环化合物富集培养的微生物多样性
Diversity of Microbe in the Intestinal Tract of Camelus bactrianus Enriched by Three Kinds of Nitrogen Heterocyclic Compounds
摘要
Abstract
To explore the biodiversity of bacteria degrading potentially heterocyclic compounds in the intestinal tract of camel (Ca-melus bactrianus),we took pyridine,quinolone and indole as the only carbon and nitrogen resources,and used the five generations of enrichment culture method to cultivate the camel rumen bacteria,and used high-throughput sequencing (Illumina Miseq) to sequence the total DNA of the five generations.The results showed that Proteobacteria,Actinobacteria,Planctomycetes,Bacteroidetes and Firmicutes constituted the five categories of bacteria with the highest abundance in the intestinal tract of camel which degraded heterocyclic compounds.The dominant bacteria for degrading pyridine belong to Bacillus,Sphingobacterium,Ochrobactrum and Pseudomonas,for degrading quinolone belong to Bacillus,Corynebacterium and Lysinibacillus,and for degrading indole belong to Sphingobacterium,respectively.However,compared with the original samples in the intestinal tract,the dominant bacteria groups enriched in the cultures of five generations obviously changed.This suggest that the intestinal tract of camel has the bacteria degrading heterocyclic compounds,and the involved species are different for degradation of pyridine,quinolone and indole.关键词
消化道/骆驼/吡啶/喹啉/吲哚/生物多样性Key words
digestive tract/Camelus bactrianus/pyridine/quinolone/indole/biodiversity分类
生物科学引用本文复制引用
唐琴,郑李娟,庄伟伟,曾献春..骆驼肠道内3种含氮杂环化合物富集培养的微生物多样性[J].厦门大学学报（自然科学版）,2017,56(4):517-524,8.基金项目
国家自然科学基金(31160028) （31160028）
国家自然科学基金委员会-新疆维吾尔自治区人民政府联合基金(U1503101) （U1503101）
四川省教育厅重点项目(17ZA0105) （17ZA0105）
成都医学院校基金项目(CYZ15-06) （CYZ15-06）
新疆师范大学博士科研启动基金(XJNUBS1607) （XJNUBS1607）
新疆维吾尔自治区百名博士引进计划(BS2016008) （BS2016008）
