畜牧兽医学报2017，Vol.48Issue(7)：1191-1201,11.DOI:10.11843/j.issn.0366-6964.2017.07.003
藏鸡FTO基因表达的时空特性及与肌内脂肪含量的相关分析
Temporal-Spatial Characteristics of FTO Gene Expression in Tibetan Chicken and Its Correlation with Intramuscular Fat Content
摘要
Abstract
The aim of this study was to obtain the sequence of FTO gene of Tibetan chicken,analyze its sequence by bioinformatics,clarify its temporal-spatial expression characteristics,and reveal the relationship between the expression level of FTO gene and intramuscular fat content (IMF) at different developmental stages.Healthy Tibetan chickens in various ages(1-210 days of age) were selected,the tissue samples of heart,liver,spleen,lung,kidney,breast muscle,leg muscle and subcutaneous fat were collected for the total RNA extraction.The Reverse Transcription PCR(RT-PCR) was used to clone Tibetan chicken FTO gene sequence,the real-time quantitative PCR(qPCR) was used to detect the expression level of FTO gene in different tissues and developmental stages,and the correlation between the FTO mRNA expression and intramuscular fat content was analyzed.The results showed that the sequence of Tibetan chicken FTO gene was 1 585 bp (GenBank accession number:KY366175),containing 1 524 bp CDS,35 bp 5'UTR and 26 bp 3'UTR,encoding 507 amino acids with a N-terminal domain and a C-terminal domain.There were 6 amino acid mutations between Tibetan chicken and Gallus gallus FTO amino acid sequences.FTO gene of Tibetan chicken was widely expressed in different tissues.The expression level of FTO gene was the highest in spleen,which was significantly higher than other tissues (P＜0.01).FTO gene also kept a high expression level in adipose and lung tissues.The expression pattern of Tibetan chicken FTO gene in breast muscle and leg muscle were different,the expression level in breast muscle decreased gradually with the growth of Tibetan chicken,while in the leg muscle,the expression level increased first and then descended with the increasing age.The expression levels of Tibetan chicken FTO gene in different muscle tissues and different developmental stages presented diverse relationships with IMF content.The expression level of FTO in breast muscle of Tibetan chicken was negatively correlated with IMF content,and this correlation in hens was significantly stronger (P＜0.05).In leg muscle,the expression level of FTO gene in cocks was positively correlated with IMF content(in period of 119-210 days,r=0.601,P＜0.05),while in hens this relationship was opposite.The results suggest that FTO gene may play an important role in intramuscular fat deposition during the growth and development stage of Tibetan chicken.These results will facilitate the research on the structure and function of FTO gene in Tibetan chickens.关键词
藏鸡/FTO基因/组织表达/时序表达/肌内脂肪含量Key words
Tibetan chickens/fat mass and obesity associated gene/tissue expression/temporal expression/intramuscular fat content分类
农业科技引用本文复制引用
林森,林亚秋,朱江江,徐亚欧,王永..藏鸡FTO基因表达的时空特性及与肌内脂肪含量的相关分析[J].畜牧兽医学报,2017,48(7):1191-1201,11.基金项目
四川省科技支撑计划“十三五”育种攻关项目(2016NYZ0043) （2016NYZ0043）
