畜牧兽医学报2017，Vol.48Issue(7)：1229-1240,12.DOI:10.11843/j.issn.0366-6964.2017.07.007
牛体外受精胚胎抗氧化相关的长链非编码RNA表达谱
Long Non-Coding RNA Profiling in Bovine Embryos Treated with Glutathione during in vitro Culture
摘要
Abstract
To reveal the effects of GSH supplemented in culture medium on lncRNA profiling of bovine embryos,the IVF embryos at 8-16-cell and morula stages were collected for high-through-put RNA sequencing (RNA-seq).Then co-expression analysis and cis-targeted genes prediction of differentially expressed lncRNAs (DElncRNAs),Gene ontology and KEGG pathway enrichment analysis of differentially expressed genes (DEGs) were performed.Additionally,qRT-PCR of 8 DElncRNAs was used to confirm the accuracy and validity of RNA-seq data.A total of 4 273 lncRNAs were obtained,and the qRT-PCR results confirmed the expression pattern of DElncRNAs from RNA-seq.Among those lncRNAs,59 were differentially expressed.Among them,23 DElncRNAs were up-regulated in control embryos while down-regulated in treated embryos,36 DElncRNAs were down-regulated in control embryos while up-regulated in treated embryos.In control group(no GSH),59 DElncRNAs were co-expressed with 754 DEGs which were involved in 47 GO terms and enriched in 5 pathways.In treated group,the co-expressed DEGs were 2 782 involving in 48 terms and participating in 13 pathways.Furthermore,145 neighbor genes of 23 DElncRNAs and 154 neighbor genes of 36 DElncRNAs were participated in 35 and 43 GO terms,respectively.DEGs co-expressed with 14 lncRNAs were enriched in GSH metabolism pathways.Conclusively,GSH treatment could change the lncRNA profiling and improve the development of bovine IVF embryos.关键词
谷胱甘肽/长链非编码RNA/转录组测序/共表达分析/靶基因Key words
glutathione/lncRNA/RNA-seq/co-expression analysis/target gene分类
农业科技引用本文复制引用
郭芹芹,李凤,孙尉峻,郝海生,刘岩,赵学明,庞云渭,朱化彬,杜卫华..牛体外受精胚胎抗氧化相关的长链非编码RNA表达谱[J].畜牧兽医学报,2017,48(7):1229-1240,12.基金项目
中国农业科学院科技创新工程“家畜胚胎工程与繁殖创新团队”(ASTIP-IAS06-2017) （ASTIP-IAS06-2017）
国家科技支撑项目(2012BAD12B01-2) （2012BAD12B01-2）
