畜牧兽医学报2017，Vol.48Issue(7)：1281-1287,7.DOI:10.11843/j.issn.0366-6964.2017.07.012
蓝舌病病毒7型毒株的分离与分子鉴定
Isolation and Identification of Bluetongue Virus Serotype 7 Field Isolate
摘要
Abstract
In 2014,cattle blood samples were collected from Guangdong province and vector-borne pathogens contained in them were detected in the present study.Samples which were detected as BTV positive by quantitative reverse transcription polymerase chain reaction (qRT-PCR) were isolated and identified.Embryonated chicken eggs were subsequently inoculated and their livers were harvested to passage on C6/36 and BHK-21 cells,respectively.Extensive cytopathic changes were observed.A subsequent qRT-PCR assay target with VP 7 gene and SEM observation results further confirmed the presence of BTV,and named as GD/ST2014.In addition,VP2 gene fragment was used for genotyping.And combined with VNT test results,the genotype of the isolate obtained in the present study were identified as BTV-7.This was the first report that bluetongue virus serotype 7 field isolate was isolated from cattle in China.关键词
蓝舌病病毒/分离/鉴定/血清型/序列分析Key words
bluetongue virus/isolation/identification/serotype/sequence analysis分类
农业科技引用本文复制引用
吕敏娜,李华春,陈琴苓,杨恒,孙铭飞,李娟,林栩慧,张健騑,廖申权,戚南山,吴彩艳..蓝舌病病毒7型毒株的分离与分子鉴定[J].畜牧兽医学报,2017,48(7):1281-1287,7.基金项目
公益性行业(农业)科研专项(201303035) （农业）
广东省科技计划项目(2015A020209075 （）
2015A020209084) （）
