畜牧兽医学报2017，Vol.48Issue(7)：1306-1313,8.DOI:10.11843/j.issn.0366-6964.2017.07.015
鸭源鸡杆菌ompW基因缺失菌株的构建及其药物敏感性分析
Construction of ompW Gene Deletion Strain of Gallibacterium anatis and Its Susceotibility to Antibacterial Agents
摘要
Abstract
The study was conducted to explore the role of ompW gene in the drug resistance of Gallibacterium anatis (G.anatis).The ompW gene knock-out mutant of G.anatis was constructed by natural transformation.The competent cells of G.anatis were induced following transfer of cells to M-Ⅳ medium from colonies on a blood agar plate.Then,transformation was performed by incubating the M-Ⅳ-compentent cells together with linear DNA fragment consisting of homologous arms and cmpr gene.The transformants were identified by using PCR,SDS-PAGE and Western blot analysis.The MIC-determinations of 12 kinds of antibacterial agents were performed for both wild type and mutant AompW G.anatis using Mueller Hinton-Ⅱ.The ompW gene deletion strain AompW was successfully constructed.Comparing with wild type G.anatis,the MICs of terramycin and tetracycline for AompW were decreased to 1/8-fold (16/128) and 1/32-fold (4/128),respectively.The MICs of other drugs in this study showed no obvious change.In conclusion,ompW deletion strain of G.anatis was constructed by natural transformation for the first time in this study.OmpW plays an important role in the formation of tetracyclines resistance phenotype of G.anatis strains.关键词
鸭源鸡杆菌/OmpW/突变菌株/抗菌药物/MICKey words
Gallibacterium anatis/OmpW/mutants/antibacterial agents/MIC分类
农业科技引用本文复制引用
彭志锋,崔丹丹,杨霞,敬文宪,王忠田,王坤芃,王川庆..鸭源鸡杆菌ompW基因缺失菌株的构建及其药物敏感性分析[J].畜牧兽医学报,2017,48(7):1306-1313,8.基金项目
国家自然科学基金(31302090) （31302090）
河南省自然科学基金(162300410153) （162300410153）
河南省高校科技创新团队与支持计划资助项目(14IRTSHN015) （14IRTSHN015）
